Paracellular and transmembrane permeability of the small intestine in newborns
I. A. Bavykina,
A. A. Berdnikov,
A. V. Kubyshkina,
A. A. Zvyagin,
S. V. Belmer,
A. I. Khavkin,
S. V. Barannikov,
K. A. Berdnikova https://doi.org/10.31146/1682-8658-ecg-238-6-32-38
Abstract
In order to determine the concentration of Claudin-2 and I-FABP to clarify the mechanisms of intestinal permeability in newborns, 115 patients (gestational age - 33-41 weeks (Me 38.0 [36.0-39.0]) aged up to 10 days were examined. The first group (n=80) consisted of full-term infants (Me 39.0 [38.0; 40.0] weeks); the second included 35 premature infants (Me 35.0 [34.5; 36.0] weeks). The ELISA method was used to determine the concentrations of Claudin-2 and I-FABP, as well as to assess the basic indicators of nutritional status. The statistical analysis was performed using the StatTech v. 4.3.2 program (Russia). The Kolmogorov-Smirnov (n≥50) and Shapiro-Wilk (n≤50) criteria, the Mann-Whitney U-test and the Kraskel-Wallis criterion, and the Spearman rank correlation coefficient were used. The differences were considered statistically significant at p<0.05. As a result of the study, it was found that the concentration of Claudin-2 in the total cohort of examined children was Iu 3,434 ng/ml [1,198-7,866], the level of I-FABP in the sample was Iu 1,134 ng/ml [0.854-1,846]. The markers had a direct relationship (rs=0.44, p<0.001). It was found that the level of Claudin-2 in premature infants is higher than in full-term infants (Me 8,124 ng/ml [6,448-13,676] VS Me 1,508 ng/ml [1,004-4.33]) (p<0.001). There is an inverse correlation between the Claudin-2 value and gestational age (rs= -0.507, p<0.001), weight (rs= -0.335, p<0.001) and body length (rs= -0.277, p=0.003), head circumference (rs= -0.316, p<0.001). The concentration of I-FABP in the blood serum in children of group 1 was Me 1.13 ng/ml [0.796-1.911], in premature infants - Me 1.134 ng/ml [1.05-1.614]. This indicator did not differ in patients in the study groups (p=0.440) and had no relation to gestational age (rs= -0.176, p=0.059), body weight (rs= -0.015, p=0.874), body length (rs=0.072, p=0.448) and head circumference (rs=0.023, p=0.805). There was no correlation between the nature of breastfeeding, antibacterial therapy, and Claudin-2 and I-FABP in blood serum (p>0.05).
About the Authors
I. A. Bavykina
Voronezh State Medical University named after N.N. Burdenko
Russian Federation
Russian Federation
A. A. Berdnikov
Voronezh State Medical University named after N.N. Burdenko
Russian Federation
Russian Federation
A. V. Kubyshkina
Voronezh State Medical University named after N.N. Burdenko
Russian Federation
Russian Federation
A. A. Zvyagin
Voronezh State Medical University named after N.N. Burdenko
Russian Federation
Russian Federation
S. V. Belmer
N.I. Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
A. I. Khavkin
Research Clinical Institute of Childhood, Ministry of Health of the Moscow Region; Belgorod State Research University. Ministry of Science and Higher Education of the Russian Federation
Russian Federation
Russian Federation
S. V. Barannikov
Voronezh State Medical University named after N.N. Burdenko
Russian Federation
Russian Federation
K. A. Berdnikova
Voronezh State Medical University named after N.N. Burdenko
Russian Federation
Russian Federation
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Bavykina I.A., Berdnikov A.A., Kubyshkina A.V., Zvyagin A.A., Belmer S.V., Khavkin A.I., Barannikov S.V., Berdnikova K.A. Paracellular and transmembrane permeability of the small intestine in newborns. Experimental and Clinical Gastroenterology. 2025;(6):32-38. (In Russ.) https://doi.org/10.31146/1682-8658-ecg-238-6-32-38
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