Morphological, ultrastructural and brightness quantitative characteristics of Helicobacter pylori electronic microscopic images
https://doi.org/10.31146/1682-8658-ecg-193-9-73-77
Abstract
Using an original computer program, a quantitative characteristic of the structural features of the cultures of two reference strains of Helicobacter pylori, identified by transmission electron microscopy, was performed. The results obtained made it possible to establish morphological, ultrastructural and brightness differences between individual bacterial cells of the studied strains. The proposed program, compiled in accordance with the requirements of computer vision technology, makes it possible to detect differences in the structure of bacterial cells that are not detected by visual assessment, and also opens up the possibility of studying the phenotypic heterogeneity of isogenic populations of Helicobacter pylori and its pathogenic significance.
About the Authors
V. G. Zhukhovitsky
Gamaleya National Research Centre for Epidemiology & Microbiology of the Ministry of Public Health; Russian Medical Academy of Continuing Professional Education (RMANPO), Ministry of Public Health
Russian Federation
Russian Federation
Vladimir G. Zhukhovitsky - MD, PhD, Head of the Laboratory of indication and ultrastructural analysis of microorganisms, Scopus Author ID: 57194386794, WoS ID: AAH-9536–2020.
Moscow, Gamaleya str., h. 18, 123098; Moscow, Barrikadnaya str., h. 2/1, build. 1, 125993
S. O. Navolnev
Gamaleya National Research Centre for Epidemiology & Microbiology of the Ministry of Public Health
Russian Federation
Russian Federation
MD, PhD, Senior Scientist of the Laboratory of indication and ultrastructural analysis of microorganisms.
Moscow, Gamaleya str., h. 18, 123098
N. V. Shevlyagina
Gamaleya National Research Centre for Epidemiology & Microbiology of the Ministry of Public Health
Russian Federation
Russian Federation
MD, PhD, Senior Scientist of the Laboratory of indication and ultrastructural analysis of microorganisms.
Moscow, Gamaleya str., h. 18, 123098
References
1. Leja M., Grinberga-Derica I., Bilgilier C., Steininger C. Review: epidemiology of Helicobacter pylori infection. Helicobacter. 2019, No. 24, Suppl. 1: e12635. DOI: 10.1111/hel.12635.
2. Chmiela M., Kupcinskas J. Pathogenesis of Helicobacter pylori infection. Helicobacter. 2019, No. 24, Suppl. 1: e12638. DOI: 10.1111/hel.12638.
3. Amieva M., Peek R. M. Pathobiology of Helicobacter pylori-induced gastric cancer. Gastroenterology. 2016;150(1):64-78. DOI: 10.1053/j.gastro.2015.09.004.
4. Harris J. R. Transmission electron microscopy in molecular structural biology: a historical survey. Arch. Biochem. Biophys. 2015, Vol. 581, pp. 3-18. DOI: 10.1016/j.abb.2014.11.011.
5. Shapiro L., Stockman J. Computer vision (translated from English). Moscow. “BINOM - Knowledge Laboratory”. 2006, 752 p. (In Russ)
6. Didenko L.V., Navolnev S. O. A program for the analysis of biological micro-objects in images obtained from an electron microscope. Scientific session of NRNU MEPhI-2012, volume 1. Innovative nuclear technologies. High technologies in medicine. Moscow. NRNU MEPhI Publ. 2012, 286 P.
7. Zhukhovitsky V.G., Smirnova T. A., Shevlyagina N. V., Korzheva I. Yu., Didenko L. V., Gintsburg A. L. Helicobacter pylori BIOFILM IN VITRO AND IN VIVO. Experimental and Clinical Gastroenterology. 2017;(5):14-19. (In Russ.) DOI: 10.31146/1682-8658-ecg-141-5-14-19.
8. Tizro P., Choi C., Khanlou N. Sample preparation for transmission electron microscopy. Methods Mol. Biol. 2019, Vol. 1897, pp. 417-424. DOI: 10.1007/978-1-4939-8935-5_33.
9. Navolnev S. O. Development of a computer program for quantitative assessment of the digital image of bacteria obtained from a transmission electron microscope. Bacteriology. 2017;2(2):20-23. DOI: 10.20953/2500-1027-2017-2-20-23.
10. Ackermann M. A functional perspective on phenotypic heterogeneity in microorganisms. Nat. Rev. Microbiol. 2015;13(8):497-508. Doi: 10.1038/nrmicro3491.
Review
For citations:
Zhukhovitsky V.G., Navolnev S.O., Shevlyagina N.V. Morphological, ultrastructural and brightness quantitative characteristics of Helicobacter pylori electronic microscopic images. Experimental and Clinical Gastroenterology. 2021;(9):73-77. (In Russ.) https://doi.org/10.31146/1682-8658-ecg-193-9-73-77
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