Bactericidal effect of low-temperature plasma against Helicobacter pylori in vitro
Abstract
The purpose of this work was to evaluate the bactericidal effect of helium plasma on Helicobacter pylori in vitro. Helium plasma was generated using a high-voltage generator with a gas flow rate of 2.5 l/min; the distance to the sample surface was 10 mm. A spectral assessment of the plasma torch was carried out, and the rate of accumulation of nitric oxide in the irradiated medium was also determined. To study the bactericidal effect of NTP, H. pylori was seeded onto Petri dishes and plasma treated for 15, 30, 60, 120, 300 s. After incubation, the diameter of the growth inhibition zones was measured. It was established that cold plasma had a bactericidal effect on H. pylori in vitro, this effect was dose-dependent and could be due to the complex effect of ultraviolet radiation, the formation of active nitrogen and oxygen particles and charged particles. Thus, cold plasma can be used for decontamination of biomedical equipment (gastroscopes) and, when modifying a source, can be a promising method for the eradication of H. pylori in vivo, including antibiotic-resistant strains.
About the Authors
V. G. Zhukhovitsky
“Gamaleya National Research Centre for Microbiology and Immunology”, Ministry of Public Health of Russian Federation; “Sechenov The First Moscow Medical University”, Ministry of Public Health of Russian Federation
Russian Federation
Russian Federation
M. V. Kazakova
“Gamaleya National Research Centre for Microbiology and Immunology”, Ministry of Public Health of Russian Federation
Russian Federation
Russian Federation
E. V. Sysolyatina
“Gamaleya National Research Centre for Microbiology and Immunology”, Ministry of Public Health of Russian Federation; “Moscow Institute of Physics and Technology (State Research University)”
Russian Federation
Russian Federation
R. A. Loleit
“Kinetika Co., Ltd”
Russian Federation
Russian Federation
A. Ya. Lavrikova
“Gamaleya National Research Centre for Microbiology and Immunology”, Ministry of Public Health of Russian Federation
Russian Federation
Russian Federation
A. V. Sofronov
“Kinetika Co., Ltd”
Russian Federation
Russian Federation
S. A. Ermolaeva
“Gamaleya National Research Centre for Microbiology and Immunology”, Ministry of Public Health of Russian Federation; “Moscow Institute of Physics and Technology (State Research University)”
Russian Federation
Russian Federation
Review
For citations:
Zhukhovitsky V.G., Kazakova M.V., Sysolyatina E.V., Loleit R.A., Lavrikova A.Ya., Sofronov A.V., Ermolaeva S.A. Bactericidal effect of low-temperature plasma against Helicobacter pylori in vitro. Experimental and Clinical Gastroenterology. 2019;(3):51-57. (In Russ.)
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