A complex combination of external factors (stress), host immune status and virulence factors determines the susceptibility and severity of the outcome of H. pneumonia infection and associated pathology. H.pylori and intestinal-brain axis relationships are bi-directional and affect the infection process and neuroendocrine immunological response of the host organism, including changes in secretory and motor functions of the digestive tract, modification of visceral sensitivity and cognitive functions. The effect of H.pylori on intestinal-brain axis is based on direct neurotoxic action, micronutrient deficiency, the activation of inflammatory processes in the epithelium, destruction of the barrier function, destabilization of the blood-brain barrier and the systemic action of pro-inflammatory cytokines.

The study included 34 patients (15 men and 19 women, average age 56,7±1,46). The test was divided into three groups, with the additional inclusion criterion of I and II groups was the use of a patient NSAIDs for at least 30 calendar days prior to the survey. A study of strains of Helicobacter pylori (HP) in gastric mucosa of adult patients receiving non-steroidal anti-inflammatory drugs (NSAIDs). HP genotyping was performed by PCR. In the General structure of the studied biopsy specimens was often identified genotype vac A s1/m1, cag A+; iceA1 (7 cases out of 34 (of 20.58 %), p<0.05). The distribution of genotypes in the clinical groups were quite heterogeneous, the Results of the conducted studies, despite some heterogeneity, indicate the presence of statistically significant differences depending on the presence of erosive-ulcerative changes and factors of NSAIDs.

The aim of research was to evaluate the effectiveness of the effect of eradication therapy on the cytokine status of gastric juice in patients with chronic non-atrophic gastritis (HNG) and duodenal ulcer (duodenal ulcer) associated with Helicobacter pylori. Materials and methods. Clinical observations and laboratory-instrumental studies were performed in 95 patients aged 20 to 55 years with HNG and duodenal ulcer with determination of cytokine content of IL-1β, IL-6 and TNF-α in the fasting portion of gastric juice. Results. The most pronounced decrease in the content of proinflammatory cytokines in gastric juice on the background of treatment was found in patients of the 1st group who received combined therapy according to the scheme omeprazole + + clarithromycin + amoxicillin. Analysis of the content of proinflammatory cytokines (IL-1β, IL-6 and TNF-α) in gastric juice in patients with acute exacerbation showed that their concentration in all 8 patients after the course of therapy exceeded the norm (P <0.05) and was IL -1β - 30,30 + 1,15 pg / l, IL-6-10,4 + 0,83 pg / l and TNF-α - 32,5 + 1,13 pg / l. At the same time, the level of proinflammatory cytokines in gastric juice correlated with the degree of dissemination of H. pylori in the mucosa of the gastroduodenal zone. Conclusion. Helicobacter pylori infection in inflammation and ulceration in the mucous membrane of the stomach and duodenum, possibly in addition to other mechanisms, affects the activation of pro-inflammatory cytokines (IL-1beta, IL-6, TNF-alpha) in gastric juice. Incomplete eradication of H. pylori after treatment during clinical endoscopic remission in patients with duodenal ulcer in the vast majority of cases is accompanied by the preservation of an increased level of pro-inflammatory cytokines in gastric juice, which may be one of the reasons for the relapse of the disease.