Humoral immune response to lipoteichoic acids of Clostridium difficile in patients with inflammatory bowel disease
https://doi.org/10.31146/1682-8658-ecg-160-12-25-32
Abstract
Aim. To evaluate the IgA, IgM and IgG to lipoteichoic acids (LPA) of Clostridium difficile (C. difficile) in inflammatory bowel disease (IBD). Materials and Methods. We prospectively included 147 patients with IBD [92 with ulcerative colitis (UC) and 55 with Crohn’s disease (CD)] and 30 healthy volunteers. The concentration of IgA, IgM and IgG to C. difficile LPA in blood was determined by enzyme immunoassay method. Results. The level of IgA to LPA of C. difficile was higher in active IBD - 0,12 [0,06; 0,19] mkg/ml, p<0,05) and in remission (0,12 [0,07; 0,18] mkg/ml) compared with in control group - 0,009 [0,005; 0,01] mkg/ml. In active CD the studied IgA were higher than in control group (p<0,01). The level of IgM to LPA of C. difficile was higher in IBD exacerbation (4,75 [2,48; 7,45 mkg/ml; p<0,001), in remission (4,69 [2,65; 7,85] mkg/ml; p<0,05) than in healthy volunteers (2,4 [1,5; 4,08] mkg/ml; p<0,01). The changes in UC and CD were identical. The studied IgG was higher in active IBD (4,17 [1,34; 6,55] mkg/ml; p<0,0001) and in remission (3,39 [1,42; 5,81] mkg/ml; p<0,01) than in control group (1,0 [0,89; 1,94] mkg/ml). The changes were similar in UC and CD. The levels of studied IgA and IgG in healthcare workers among our control group was higher than in the persons not related to medicine. Conclusions. The levels IgA, IgM and IgG to C. difficile LPA in IBD was significant increase compared with in healthy volunteers. The increase of antibodies was characteristic for active and inactive IBD.
About the Authors
D. . Abdulganieva
Federal State Budgetary Educational Institution of Higher Education “Kazan State Medical University” of the Ministry of Healthcare of the Russian Federation, department of hospital therapy
Russian Federation
Russian Federation
D. . Mukhametova
Federal State Budgetary Educational Institution of Higher Education “Kazan State Medical University” of the Ministry of Healthcare of the Russian Federation, department of hospital therapy
Russian Federation
Russian Federation
O. . Zinkevich
Kazan State Medical Academy - Branch Campus of the Federal State Budgetary Educational Institution of Further Professional Education “Russian Medical Academy of Continuous Professional Education” of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
N. . Saphina
Kazan State Medical Academy - Branch Campus of the Federal State Budgetary Educational Institution of Further Professional Education “Russian Medical Academy of Continuous Professional Education” of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
M. . Koporulina
Kazan State Medical Academy - Branch Campus of the Federal State Budgetary Educational Institution of Further Professional Education “Russian Medical Academy of Continuous Professional Education” of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
A. . Odintsova
State Autonomous Healthcare Institution “Republican Clinical Hospital of the Ministry of Health of the Republic of Tatarstan”
Russian Federation
Russian Federation
Review
For citations:
Abdulganieva D., Mukhametova D., Zinkevich O., Saphina N., Koporulina M., Odintsova A. Humoral immune response to lipoteichoic acids of Clostridium difficile in patients with inflammatory bowel disease. Experimental and Clinical Gastroenterology. 2018;(12):25-32. (In Russ.) https://doi.org/10.31146/1682-8658-ecg-160-12-25-32
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