MORPHOFUNCTIONAL STATE OF THE LIVER WITH A LONG-TERM INFLUENCE OF LOW-FREQUENCY ACOUSTIC FLUCTUATIONS

Purpose of the study. To study the effect of prolonged exposure to low-frequency acoustic fluctuations on the morphofunctional state of the liver. Materials and methods. The experiments were performed on 48 white male rats. Three experimental groups of animals were subjected to a daily one-hour noise impact in the low-frequency range with a sound pressure level of 110 dB according to the schedule: 5 days a week with two-day breaks for 1, 3 and 6 weeks. Three control groups were kept in silence. In the blood plasma, aminotransferase, gamma glutamyltranspptedase, alkaline phosphatase, total bilirubin, total protein were studied. Morphological study of liver preparations stained with hematoxylin and eosin was performed under light microscopy. Results. It was found that prolonged exposure to low-frequency acoustic fluctuations was accompanied by cytolytic syndrome and an increase in total blood lipids, without disturbing the protein-synthetic, pigmentary function of the liver. The activity of aminotransferases in animal experimental groups was 20-21 % (p≤0.5) higher than the control ones after the first week of exposure to low-frequency acoustic fluctuations. An increase in the duration of the action of LFN did not lead to an increase in the cytolytic syndrome: after a 6-week exposure, the level of alanineaminotransferase and aspartateaminotransferase was higher than the control ones by 27 % and 25 % (p≤0.5). There was also an isolated increase in alkaline phosphatase after the third week of exposure to low-frequency acoustic fluctuations with a relative increase to 23 % (p≤0.5). Morphological changes in the liver were nonspecific in nature and were represented by focal necrotic changes and a violation of intrahepatic hemodynamics with an extension of the sinusoidal channel and increased venous plethora. Conclusion. The performed studies indicate a non-specific effect of low-frequency acoustic fluctuations on the morphofunctional state of the liver. In this experimental model, reproducing the mode of professional activity, the liver state was characterized by the predominant development of cytolytic syndrome and compensatory morphological shifts, which do not lead to severe violations of liver functions. On the basis of this, it can be assumed that changes in the liver in persons who have been exposed to low-frequency acoustic fluctuations for a long time in professional activity will be subclinical.

low-frequency acoustic fluctuations, liver, experimental animals

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