Rs1800795 IL6 and rs5275 PTGS2 gene polymorphisms: role in the development of acute biliary and alcoholic-alimentary pancreatitis

The aim: identifying the role of single nucleotide polymorphism (SNP) carrier rs1800795 (-174G/C) of the interleukin-6 IL6 gene and rs5275 (+ 8473T/C) of the cyclooxygenase-2 PTGS2 gene in the development of acute pancreatitis. Materials and methods: The case-control study involved 296 people diagnosed with acute pancreatitis (OP). There were 124 (41.89%) males and 172 (58.11%) females. By age, patients ranged from 24 to 83 years old, the average age was 44,3±12,6 years. Patients with mild severity OP - 103 (34.79%) people, moderate severity OP - 110 (37.16%) people, severe severity OP - 83 (28.05%) people. The control group amounted to 78 people. In real time determined by the PCR method the frequency of occurrence of ONP rs1800795 (-174G/C) a gene of IL6 and ONP rs5275 interleukin-6 (+ 8473T/C) a gene of PTGS2 cyclooxygenase-2 at sick OP with varying severity. Statistical data processing was carried out using the MS Excel for Windows program and the STATISTICA 10.0 software package. Results: When comparing alleles in patients of all grades with the control group, carriage as a risky variant allele G (rs1800795 (-174G/C) rs1800795 (interleukin-6 IL6 gene) (OR 2,334; 95% CI 1,611-3.381; p < 0.001) and homozygous GG (OR 6.713; 95% CI 2,957-15,242; p < 0.001) genotypes can be considered as a risk factor for the development of acute pancreatitis. Comparison of alleles in patients with severe OP severity and control group allowed to establish that carriage as a risky variant allele of G (rs1800795 (-174G/C) rs1800795 (IL-6) gene (OR 2.320; 95% CI 1.435-3.750; p < 0.001) and homozygous GG (OR 7.607; 95% CI 3.023-19.146; p < 0.001) of the genotype can be considered as a risk factor for the development of severe acute pancreatitis. When comparing alleles in patients of all grades with control, carrying a risky variant G allele of the PTGS2 gene (COX-2) (OR 1.588; 95% CI 1.063-2.282; p = 0.023), is a risk factor for the development of OP. When comparing alleles in patients with severe severity of OP and control group: carrying a risky variant allele G (OR 1.813; 95% CI 1.117-2.942; p = 0.016), can be considered as a risk factor for the development of a severe course of acute pancreatitis. Conclusion: Thus, in residents of Krasnoyarsk, the carriage of both the rs1800795 (-174G/C) IL6 gene and the rs5275 (+ 8473T/C) PTGS2 gene can serve as a predictor of the development of OP and severe disease

acute pancreatitis, genetic predictors, severe acute pancreatitis, gene polymorphism, interleukins

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