Promising bacteria-producers of biologically active substances for agriculture and food industry

Purpose. Study of biotechnological potential of strains of the BIOTROF company collection using whole-genome NGS sequencing. Material and methods. The genome of 6 bacterial strains of the genera Bacillus, Lactobacillus and Streptococcus from the collection of BIOTROF LLC (St. Petersburg) was analyzed. Bioinformatics data analysis included processing in software packages of tools and databases RAST, KEGG Pathway, antiSMASH 6.0, Norine, CAZy, PHASTER, VirulenceFinder and ResFinder. Results. The analysis showed that the studied strains had genes associated with the synthesis of a large number of antimicrobial substances of various natures and spectrums of action, enzymes, vitamins, amino acids, genes of resistance to aggressive environmental factors and other properties that determine the high potential of their application in various industries. The use of strains of the genus Bacillus and Enterococcus may be more preferable for the production of biopreparations for plant growing (stimulation of plant growth and protection, bioremediation) and animal husbandry (regulation of the gastrointestinal microbiome, increased immunity, detoxification, increased availability of nutrients) due to a wider range of genes associated with adaptation, enzymatic activity, antimicrobial protection, synthesis of vitamins and amino acids. Lactobacillus plantarum 600, Bacillus subtilis 111, Bacillus mucilaginosus 159 in the genome have determinants of osmotolerance and synthesis of antimicrobial compounds, which determines their potential as preservatives for feed, and Streptococcus thermophilus L has genes for the synthesis of exopolysaccharides necessary for the production of fermented milk products. The genome of all the studied strains did not contain elements that contribute to the formation of toxic and virulent properties of the strains. Conclusion. The studied strains have been shown to have high potential for use in crop production, livestock farming and the food industry.

biopreparation, bacterial strains, collection of biotechnological strains, whole genome sequencing, metabolic potential

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