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Experimental and Clinical Gastroenterology

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Study of liver tissue with topical application of new spongy hemostatic agents in experiment

https://doi.org/10.31146/1682-8658-ecg-231-11-119-127

Abstract

Research Objective: To study the histological features of the reaction of liver tissue in an in vivo experiment when modeling various types of liver damage and using new samples of local hemostatic agents Materials and methods. The research materials used were samples of new spongy polymer hemostatic agents (based on marine collagen, in different weight ratios with sodium salt of carboxymethylcellulose (three types of samples containing 15%, 25%, 50% collagen), as well as products used in clinical practice - hemostatic collagen sponge, Tachocomb collagen plate. The reaction of liver tissues to the use of these products in a chronic in vivo experiment was evaluated: rabbits underwent laparoscopic modeling of three variants of liver injury (tangential marginal resection of the right lobe, laceration of the middle lobe with a depth of 1.5 cm, superficial wound of the left lobe 1×1 cm), while hemostasis was performed by application or injection. In this case, hemostasis was performed by applying or injecting the tested products into the wound. The animals were taken out on the 28th day. Micro-preparations were manufactured, which were stained with hematoxylin and eosin, according to the Van Gieson method. Results. A comparison of the histological picture of connective tissue in the area of experimental liver damage under conditions of use for hemostasis of different materials: hemostatic collagen sponge, Tachocomb hemostatic plate, as well as new samples shows that, regardless of the type of liver damage (wound in the thickness of the organ, marginal injury or superficial injury), a less pronounced tissue reaction and rapid Biodegradation shows the hemostatic collagen sponge material. Conclusion. In comparison with a hemostatic collagen sponge, the use of a sample based on the sodium salt of carboxymethylcellulose with the addition of 15% collagen as a local remedy for stopping bleeding leads to a slowdown in the formation of connective tissue due to the “distraction” of macrophages to resorption of the remnants of the sodium salt of carboxymethylcellulose.

About the Authors

V. A. Lipatov
Kursk state medical university
Russian Federation


S. V. Lazarenko
Kursk state medical university
Russian Federation


A. V. Ivanov
Kursk state medical university
Russian Federation


D. A. Severinov
Kursk state medical university
Russian Federation


A. A. Denisov
Kursk state medical university
Russian Federation


A. E. Kovaleva
Kursk state medical university
Russian Federation


References

1. Evtikhov A.V., Lyubivyy E.D., Kim V.L. Treatment of severe liver trauma. Pirogov Russian Journal of Surgery. 2020;(7):89-92. (In Russ.) doi: 10.17116/hirurgia202007189.@@ Евтихов А.В., Любивый Е.Д., Ким В.Л. Клинические наблюдения тяжелых травматических повреждений печени. Хирургия. Журнал им. Н.И. Пирогова. 2020;7: 89-92. doi: 10.17116/hirurgia202007189.

2. Parkhisenko Yu.A., Vorontsov A.K., Vorontsov K.E., et al. Analysis of the results of the surgical treatment of patients with trauma of the liver. Perspectives of Science & Education. 2018; 1(31): 245-250. (In Russ.)@@ Пархисенко Ю.А., Воронцов А.К., Воронцов К.Е., и др. Анализ результатов хирургического лечения пациентов с травматическими повреждениями печени. Перспективы науки и образования. 2018; 1(31): 245-250.

3. Andreev A.A., Ostroushko A.P., Laptiyova A.Y., Glukhov A.A. Reparative liver regeneration after segmental resection (literature review). Aspirantskiy Vestnik Povolzhiya. 2018;5-6(18): 183-190. (In Russ.) doi: 10.17816/2072-2354.2018.18.3.183-190.@@ Андреев А.А., Остроушко А.П., Лаптиёва А.Ю., Глухов А.А. Репаративная регенерация печени после сегментарной резекции (литературный обзор). Аспирантский вестник Поволжья. 2018;5-6(18): 183-190. doi: 10.17816/2072-2354.2018.18.3.183-190.

4. Sung Y.K., Lee D.R., Chung D.J. Advances in the development of hemostatic biomaterials for medical application. Biomaterials research. 2021;1(25): s40824-021-00239-1. doi: 10.1186/s40824-021-00239-1.

5. Gereng E.A., Vesir I.R., Kliushina T.S. Morphological and functional characteristics of liver regeneration when using the implant made of titanium nickelide (experimental study). Crimean journal of experimental and clinical medicine. 2020; 3(10): 12-15. (In Russ.) doi: 10.37279/2224-6444-2020-10-3-12-15.@@ Геренг Е.А., Весир И.Р., Клюшина Т.С. Морфофункциональные особенности регенерации печени при использовании имплантата из никелида титана (экспериментальное исследование). Крымский журнал экспериментальной и клинической медицины. 2020; 3(10): 12-15. doi: 10.37279/2224-6444-2020-10-3-12-15.

6. Zuk I.G., Lozhko P.M., Kudlo V.V. et al. Anatomical and experimental reasoning of liver resection by hydrodissection method. Hepatology and Gastroenterology. 2023;7(1):37-42. (In Russ.) doi: 10.25298/2616-5546-2023-7-1-37-42.@@ Жук И.Г., Ложко П.М., Кудло В.В. и др. Морфологическая характеристика раны печени при ее резекции с применением некоторых методов локального гемостаза в эксперименте. Гепатология и гастроэнтерология. 2023;1(7):37-42. doi: 10.25298/2616-5546-2023-7-1-37-42.

7. Park Y.M., Seo H.I., Kim J.H. et al. Clinical application of a new hemostatic material using mussel-inspired catecholamine hemostat: A pilot study. Annals of hepato-biliary-pancreatic surgery. 2022;1(26):98-103. doi: 10.14701/ahbps.21-077.

8. Vorontsov A.K., Troshin V.P., Parkhomenko Yu.A. et al. Features of recovery of liver cells after injury in an experimental study. Modern problems of science and education. 2020; 4:90-103. (In Russ.) doi: 10.17513/spno.29941.@@ Воронцов А.К., Трошин В.П., Пархисенко Ю.А. и др. Особенности регенерации клеток печени после травмы в экспериментальном исследовании. Современные проблемы науки и образования. 2020; 4: 90-103. doi: 10.17513/spno.29941.

9. Tkachenko A.N., Savitskiy D.S., Khromov A.A. Experimental study of hemostasis at liver injuries. Journal of experimental and clinical surgery. 2021; 14(1): 47-52. (In Russ.) doi: 10.18499/2070-478X-2021-14-1-47-52.@@ Ткаченко А.Н., Савицкий Д.С., Хромов А.А. Экспериментальное изучение гемостаза при травмах печени. Вестник экспериментальной и клинической хирургии. 2021; 14(1): 47-52. doi: 10.18499/2070-478X-2021-14-1-47-52.

10. Laptiyova A. Yu., Andreev A.A., Glukhov A.A. et al.Intraoperative options to stimulate the reparative liver regeneration in the experiment. Journal of experimental and clinical surgery. 2023;4(16):294-302. (In Russ.) doi: 10.18499/2070-478X-2023-16-4-294-302.@@ Лаптиева А.Ю., Андреев А.А., Глухов А.А. и др. Интраоперационные способы стимуляции репаративной регенерации печени в эксперименте. Вестник экспериментальной и клинической хирургии. 2023; 4(16):294-302. doi: 10.18499/2070-478X-2023-16-4-294-302.

11. Simpson A., Shukla A., Brown A.C. Biomaterials for Hemostasis. Annual review of biomedical engineering. 2022;24:111-135. doi: 10.1146/annurev-bioeng-012521-101942.

12. Huang L., Liu G.L., Kaye A.D. et al. Advances in topical hemostatic agent therapies: a comprehensive update. Adv Ther. 2020;37(10):4132-4148. doi: 10.1007/s12325-020-01467-y.

13. Lipatov V.A., Lazarenko S.V., Severinov D.A. et al.Comparative analysis of efficacy of the new local hemostatic agents. Extreme Medicine. 2023; (4): 120-124. (In Russ.) doi: 10.47183/mes.2023.063.@@ Липатов В.А., Лазаренко С.В., Северинов Д.А. и др. Сравнительный анализ эффективности новых образцов местных гемостатических средств. Медицина экстремальных ситуаций. 2023;4:120-124. doi: 10.47183/mes.2023.063.

14. Huang H., Chen H., Wang X. et al. Degradable and bioadhesive alginate-based composites: an effective hemostatic agent. ACS Biomater Sci Eng. 2019;5(10):5498-5505. doi: 10.1021/acsbiomaterials.9b01120.

15. Hwang S., Na B.G., Kim M. et al. Rescue fibrin glue-infiltrating hemostasis combined with hepatorrhaphy to control intractable postoperative bleeding from the liver cut surface. Annals of hepato-biliary-pancreatic surgery. 2021;4(25):517-522. doi: 10.14701/ahbps.2021.25.4.517.


Review

For citations:


Lipatov V.A., Lazarenko S.V., Ivanov A.V., Severinov D.A., Denisov A.A., Kovaleva A.E. Study of liver tissue with topical application of new spongy hemostatic agents in experiment. Experimental and Clinical Gastroenterology. 2024;(11):119-127. (In Russ.) https://doi.org/10.31146/1682-8658-ecg-231-11-119-127

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