Morphofunctional characteristics of the liver and pancreas under the influence of the hepatoprotector “Remaxol” against the background of acute alcohol damage in rats
https://doi.org/10.31146/1682-8658-ecg-218-10-216-220
Abstract
The goal. To study the effect of hepatoprotective drug “Remaxol” on morphology of the liver and pancreas as well as on blood serum indices in rats with alcohol damage. Materials and Methods. The study was conducted on mature male white laboratory rats divided into three groups: the first (control group) - intact animals, the second and third (experimental groups) - daily part of the rats received 40 % aqueous ethanol solution (96 % ethanol in a dose of 8ml/kg weight) for 7 days, then part of the rats from the experimental group were injected “Remaxol” in a dose 5 mg/kg weight daily for 7 days. Morphological study of the liver and its macrostructure was carried out; diameters of hepatocytes, sinusoid capillaries and pancreatic acini in animals of the control and experimental groups were measured; general and biochemical blood parameters were assessed. Results. Blood tests demonstrated acute alcohol poisoning in experimental groups (leukocytosis); short-term (7 days) alcohol damage had less effect on the size of hepatocytes than on the size of pancreatic acini; pancreatic acini were more reactive on toxic influence of alcohol; in the liver and pancreas there were signs of inflammatory reaction (cell swelling, lymphocytes infiltration). Conclusion. Histological picture of the liver and pancreas of the animals with acute alcohol poisoning on the background of “Remaxol” treatment showed a decrease in the toxic effect of 40 % alcohol on hepatocytes and pancreatocytes, normalization of their morphological parameters.
About the Authors
T. G. Glushkova
Izhevsk State Medical Academy
Russian Federation
Russian Federation
S. I. Lentsova
Izhevsk State Medical Academy
Russian Federation
Russian Federation
M. A. Gorbunova
Izhevsk State Medical Academy
Russian Federation
Russian Federation
References
1. Massey V. L., Arteel G. E. Acute alcohol-induced liver injury. Front. Physio. 2012, Vol. 3, pp. 193. (in Russ.) doi: 10.3389/fphys.2012.00193.@@ Мэсси, В. Л. Острое алкогольное повреждение печени / В. Л. Мэсси и Г. Э. Артил // Фронт. Физиотерапевт. - 2012. - Том 3. - С. 193. doi: 10.3389/fphys.2012.00193.
2. Crawford J. M. Histologic findings in alcoholic liver disease. Clin.Liver Dis. 2012, Vol.16, pp. 699-716. (in Russ.) doi: 10.1016/j.cld.2012.08.004.@@ Кроуфорд, Дж. М. Гистологические данные при алкогольной болезни печени / Дж. М. Кроуфорд //Клин.печеночная дис.- 2012. - Том 16. - С. 699-716. doi: 10.1016/j.cld.2012.08.004.
3. Ilchenko L. U., Okovity S. V. Remaxol: mechanisms of action and application in clinical practice. Part 1. Archive of Internal Medicine. 2016, No. 2, pp. 16-21. (in Russ.)@@ Ильченко Л. Ю., Оковитый С. В. Ремаксол: механизмы действия и применение в клинической практике. Часть 1 / Л. Ю. Ильченко, С. В. Оковитый // Архив внутренней медицины. - 2016. - № 2. - С. 16-21.
4. Fedorenko V. N., Nadeev A. P., Pustovetova M. G. Morpho-metric study of pancreatoduodenal zone organs in acute pancreatitis and its treatment in experiment. Medical and fundamental sciences. 2011, No. 5, pp. 1-7. (in Russ.)@@ Федоренко В. Н., Надеев А. П., Пустоветова М. Г. Морфометрическое исследование органов панкреатодуоденальной зоны при остром панкреатите и его лечение в эксперименте. Медицинские и фундаментальные науки. - 2011. - № 5. - С. 1-7.
5. Vlasov A. P., Muratova T. A., Rubtsov O. Y. et al. Remaxol in the correction of functional liver disorders in acute biliary pancreatitis. Antibiotics and chemotherapy. 2015;60:5-6. (in Russ.)@@ Власов А. П., Муратова Т. А., Рубцов О. Ю. и соавт. Ремаксол в коррекции функциональных расстройств печени при остром билиарном панкреатите. Антибиотики и химиотерапия. - 2015, 60; 5-6.
6. Bogdanov A. N., Mazurov V. I. Hemolytic anemia. Bulletin of the St. Petersburg Medical Academy of Postgraduate Education. 2011, No. 3, pp. 107-114. (in Russ.)@@ Богданов А. Н., Мазуров В. И. Гемолитические анемии. / А. Н. Богданов, В. И. Мазуров // Вестник Санкт-Петербургской медицинской академии последипломного образования. - 2011. - № 3. - С. 107-114.
7. Methodological recommendations for the study of hepatoprotective activity of medicines / A. I. Vengerovsky, V. V. Udut, D. V. Reichart, A. M. Dygai. In the book “Guidelines for conducting preclinical studies of medicines”, edited by A. N. Mironov. Moscow. GrifiCo Publ., 2012. pp. 710-718. (in Russ.)@@ Методические рекомендации по изучению гепатопротекторной активности лекарственных средств / А. И. Венгеровский, В. В. Удут, Д. В. Рейхарт, А. М. Дыгай. - В кн. «Руководство по проведению доклинических исследований лекарственных средств» под ред. А. Н. Миронова. - М.: ГрифиКо, 2012. - С. 710-718.
8. Avtandilov G. G. Medical morphometry. Moscow. Medicine Publ., 1990. 26 p. (in Russ.)@@ Автандилов Г. Г. Медицинская морфометрия / Г. Г. Автандилов. - Москва: Медицина, 1990. - 26 с.
9. Shcherbakov P. L. On the question of pancreatitis. Attending Physician. Gastroenterology. 2011. № 7. (in Russ.)@@ Щербаков П. Л. К вопросу о панкреатите // Лечащий Врач. Гастроэнтерология. 2011. № 7.
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For citations:
Glushkova T.G., Lentsova S.I., Gorbunova M.A. Morphofunctional characteristics of the liver and pancreas under the influence of the hepatoprotector “Remaxol” against the background of acute alcohol damage in rats. Experimental and Clinical Gastroenterology. 2023;(10):216-220. (In Russ.) https://doi.org/10.31146/1682-8658-ecg-218-10-216-220
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