TNF and MMP1 gene polymorphisms and their association with clinical aspects of colorectal cancer

Abstract

Aim. To evaluate associations of polymorphic variants of some genes with such clinical aspects of colorectal cancer as gender, age of manifestation of the disease, localization of the tumor, the stage of the process at the time of manifestation. Materials and methods. Polymorphic variants of TNF and MMP1 genes were evaluated in 178 patients. The main group consisted of 110 patients with colorectal cancer, the control group consisted of 68 healthy volunteers. Genotyping was performed by DNA isolation from leukocytes of venous blood of the subjects followed by PCR with electrophoretic detection of the result. Description and comparison of differences in the frequencies of qualitative traits in independent groups was performed using Pearson’s χ2 criterion. Odds ratios (OR, 95% confidence interval) were also calculated for polymorphism variants that showed a correlation relationship. Differences at p<0.05 were considered statistically significant in the analysis. Results. Polymorphic variants of TNF gene (G308A) showed association with colorectal cancer in men and women, age of manifestation 50-59 and 60-69, 70 and older, localization of tumor in rectum and colon, I-III stages of tumor process. Polymorphic variants of MMP1 gene (1607 1G/2G) are associated with the development of colorectal cancer in women, age of manifestation 60-69 years old, colorectal cancer stage I-III. Conclusion. The results obtained suggest that not only risk factors influence the development of colorectal cancer, but also the human genetic status itself contributes to the development of tumors of these localizations.