The study of morphofunctional changes in the gastrointestinal tract after various operations on the colon (an experiment in rats)
D. A. Khubezov,
O. V. Zajcev,
I. S. Ignatov,
A. Y. Ogorel’cev,
Y. B. Li,
D. K. Puchkov,
S. V. Snegur,
S. S. Burmistrova,
T. I. Leukhina https://doi.org/10.31146/1682-8658-ecg-201-5-71-77
Abstract
Objective: modeling various types of surgical procedure in an experiment in rats, assessing the effect of surgery on the motility of the gastrointestinal tract (GIT).
Materials and methods: the study was carried out on 30 female Wistar rats weighing 200–250 grams, comparable in age, divided into 3 groups (10 animals each) in random order. group 1 — rats underwented to laparotomy (LT), group 2 — laparotomy, creation of a loop colostomy (LT + C), group 3 — laparotomy, a colonic single-row anastomosis (LT + A). Gastrointestinal motility was assessed using the gastrointestinal transit index (GTI), calculated by the formula: (length of the stained area of the intestine / total length of the intestine) x100, as well as by the results of a morphological study.
Results: surgery has a depressing effect on the motility of the gastrointestinal tract, while the maximum decrease in IHT was noted in the Colostomy group: 1.08±1.4% versus 2.3±3.3% in the Laparotomy group and versus 3.8±1.8% in the Anastomosis group. Comparison of the groups “Colostomy” and “Anastomosis” showed a significant difference in this feature — p=0.002.
Conclusion: modeling of various surgical procedure in rats made it possible to prove the relationship between the formation of a stoma and the inhibition of gastrointestinal motility, further study of this pattern will allow us to develop ways to prevent the development of postoperative ileus.
About the Authors
D. A. Khubezov
Ryazan State Medical University
Russian Federation
Russian Federation
Dmitriy A. Khubezov, Doctor of Science in Medical Sciences, Professor of the Department of Surgery, Obstetrics and Gynecology, Faculty of Postgraduate Education
390026, Ryazan, Vysokovol’tnaya, 9
O. V. Zajcev
Ryazan State Medical University; Ryazan State Clinical Hospital
Russian Federation
Russian Federation
Oleg V. Zajcev, Doctor of Science in Medical Sciences, Head of the Department of Surgery, Obstetrics and Gynecology, Faculty of Postgraduate Education, Head of Surgical Service
Researcher ID: R-6830–2016
390026, Ryazan, Vysokovol’tnaya, 9
390039, Ryazan, Internacional’naya, 3a
I. S. Ignatov
Ryazan State Medical University; Ryazan State Clinical Hospital
Russian Federation
Russian Federation
Ivan S. Ignatov, Ph.D. of Medical Sciences, assistant of the Department of Surgery, Obstetrics and Gynecology, Faculty of Postgraduate Education, Head of Oncology Department
390026, Ryazan, Vysokovol’tnaya, 9
390039, Ryazan, Internacional’naya, 3a
A. Y. Ogorel’cev
Ryazan State Medical University; Ryazan State Clinical Hospital
Russian Federation
Russian Federation
Aleksandr Yu. Ogorel’cev, Ph.D. of Medical Sciences, Associate Professor of the Department of Faculty Surgery with a course of anesthesiology and resuscitation, doctor of the Oncology Department
390026, Ryazan, Vysokovol’tnaya, 9
390039, Ryazan, Internacional’naya, 3a
Y. B. Li
Ryazan State Clinical Hospital
Russian Federation
Russian Federation
Yuliya B. Li, author for correspondence, doctor of the Oncology Department
Researcher ID: AAZ-9664–2021
390039, Ryazan, Internacional’naya, 3a
D. K. Puchkov
Ryazan State Medical University
Russian Federation
Russian Federation
Dmitriy K. Puchkov, Ph.D. of Medical Sciences, Associate Professor of the Department of Surgery, Obstetrics and Gynecology, Faculty of Postgraduate Education
390026, Ryazan, Vysokovol’tnaya, 9
S. V. Snegur
Ryazan State Clinical Hospital
Russian Federation
Russian Federation
Svetlana V. Snegur, Head of the Pathological department
390039, Ryazan, Internacional’naya, 3a
S. S. Burmistrova
Ryazan State Medical University
Russian Federation
Russian Federation
Svetlana S. Burmistrova, student of medical faculty, 5 year
390026, Ryazan, Vysokovol’tnaya, 9
T. I. Leukhina
Ryazan State Medical University
Russian Federation
Russian Federation
Tat’yana I. Leukhina, student of medical faculty, 4 year
390026, Ryazan, Vysokovol’tnaya, 9
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Review
For citations:
Khubezov D.A., Zajcev O.V., Ignatov I.S., Ogorel’cev A.Y., Li Y.B., Puchkov D.K., Snegur S.V., Burmistrova S.S., Leukhina T.I. The study of morphofunctional changes in the gastrointestinal tract after various operations on the colon (an experiment in rats). Experimental and Clinical Gastroenterology. 2022;(5):71-77. (In Russ.) https://doi.org/10.31146/1682-8658-ecg-201-5-71-77
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