Comparative evaluation of the application of hepatoprotector LIV-52 and prebiotic Rekicen-RD in preventive correction of experimental NSAID-hepatopathywith Nimesulide
https://doi.org/10.31146/1682-8658-ecg-176-4-115-120
Abstract
Objective: to conduct a comparative assessment of the use of the hepatoprotector and the prebiotic in NSAIDs-hepatopathy, induced by prolonged use of nimesulide using histological and immunohistochemical methods.
Materials and methods: The experiment was conducted on laboratory animals (rats), which were used to model NSAID-hepatopathy by administering nimesulide by the oral route for 21 days. Animals from the two experimental groups received a hepatoprotector and a prebiotic (simultaneously with nimesulide). The eff ect of the drugs was assessed by the results of histological examination of the liver tissue and the manifestation of the immunohistochemical expression of receptors of tumor necrosis factor α (TNFαR1) on hepatocytes. To detect receptor expression, labeled TNFR1 (polyclone) antibodies, species-specifi c to rat tissue antigens, were used.
Results: histological examination of animals from experimental groups revealed the preservation of liver tissue, which was manifested by a decrease in pathological disorders. The study of immune responses in the pathogenesis of NSAIDs-hepatopathy demonstrated that the simultaneous use of both hepatoprotector and prebiotic signifi cantly reduced the eff ect of tumor necrosis factor α on liver tissue, which was confi rmed by a low level of TNFαR1 expression in animals of experimental groups compared to manifestations of TNFαR1 expression in animals with NSAID-hepatopathy. Hepatoprotector had a more pronounced protective eff ect compared to the prebiotic.
Conclusion: studies have shown that the simultaneous use of both hepatoprotector and prebiotic with nimesulide reduces histomorphological and immune disorders in the liver tissue. Drugs can be recommended for the correction of pathological disorders of the liver tissue with prolonged use of NSAIDs.
About the Authors
L. V. Lazarenko
Perm Institute of the Federal Penal Service
Russian Federation
Associate Professor of the Department of Zootechnics, Candidate of Veterinary Sciences
125, Karpinski str., Perm, Russia, 614012
Russian Federation
Associate Professor of the Department of Zootechnics, Candidate of Veterinary Sciences
125, Karpinski str., Perm, Russia, 614012
P. V. Kosareva
Perm State National Research University
Russian Federation
Professor of the Department of Inorganic Chemistry, Chemical Technology and Technosphere Safety, Doctor of Medical Sciences
15, Bukireva str., Perm, Russia, 614990
Russian Federation
Professor of the Department of Inorganic Chemistry, Chemical Technology and Technosphere Safety, Doctor of Medical Sciences
15, Bukireva str., Perm, Russia, 614990
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Review
For citations:
Lazarenko L.V., Kosareva P.V. Comparative evaluation of the application of hepatoprotector LIV-52 and prebiotic Rekicen-RD in preventive correction of experimental NSAID-hepatopathywith Nimesulide. Experimental and Clinical Gastroenterology. 2020;174(4):115-120. (In Russ.) https://doi.org/10.31146/1682-8658-ecg-176-4-115-120
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