Association between glycation end products precursors and gut microbiota composition

Purpose of the study. To study the relationship between the gut microbiota composition and the levels of the end glycation products precursors in individuals without clinical manifestations of chronic diseases. Materials and methods. The study included 92 participants aged 25 to 76 years without clinical manifestations of severe somatic pathologies not receiving any drug therapy but with the possible presence of risk factors including metabolic disorders. All participants underwent a thorough preliminary examination, which included physical examination, clinical and biochemical blood tests, electrocardiography and treadmill test, applanation tonometry, as well as V3-V4 sequencing of variable regions of the 16S rRNA gene of gut microbiota and analysis of glyoxal and methylglyoxal levels in the blood. Results. The levels of glyoxal and methylglyoxal correlated with each other (r = 0.238, p = 0.0016). Among the clinical parameters, a high level of glyoxal in the blood was associated with an increase of systolic blood pressure (r = 0.24, p <0.002). At the same time, the correlation did not reach the level of confidence in the relationship of glyoxal with the vascular wall rigidity. The result of the analysis of the microbial composition of the gut microbiota was the discovery of relationship between an increase in the level of glyoxal with a high representation of the poorly studied family of gram-positive bacteria Mogibacteriaceae. Without the use of filtration methods of underrepresented genera, the interrelation of Fusobacteria order with a high level of methylglyoxal was also discovered, which, nevertheless, requires further study on larger cohorts. Conclusion. High levels of circulating glycation end products precursors are associated with changes in the composition of the gut microbiota, including an increase in the number of Mogibacteriaceae family.

конечные продукты гликирования, микробиом, микробиота кишечника, 16S рРНК секвенирование, жесткость сосудистой стенки, факторы риска сердечно-сосудистых заболеваний, advanced glycation end products, microbiome, gut microbiota, 16S rRNA sequencing, vascular wall stiffness, cardiovascular risk factors