The first experience of successful treatment of pseudomembranous colitis with lactobacilli auto-strains

Abstract

Aim: to imply a method of Clostridium (Clostridioides) difficile-associated infection treatment based on lactobacilli autostrains. Materials and methods: Toxin-producing vancomycin-resistant C. difficile and three types of lactobacilli in different titers were isolated from the translucent feces of a patient with recurrent C. difficile-associated infection. According to the results of the study, the strain Lactobacillus zeae was detected, which showed high antagonistic activity against C. difficile. Within 48 hours, biomass of L. zeae was accumulated. The patient was administered a suspension of lactobacilli per rectum (in the form of microclysters) every other day. Results: in a patient with severe pseudomembranous colitis, a persistent remission of the underlying disease was achieved against the background of regular injections of a suspension of Lactobacillus zeae autostrains with a high antagonistic activity against C. difficile, isolated in this patient. Conclusion: Lactobacillus transplantation is an effective and promising treatment for recurrent clostridial infection resistant to antibiotic therapy.