Genes Of The Antioxidant System Of CAT, GSTP1, GPX4 At Patients With Chronic Viral Hepatitis C

Abstract

Aims. The paper studies the polymorphism of antioxidant system genes, CAT, GSTP1, GPX4, in patients with chronic hepatitis C. Materials and methods. The paper examines 83 patients in the age from 18 to 70 with chronic hepatitis C in the reactivation phase and 30 healthy individuals. Real-time PCR methods are used to analyze polymorphic types of the studied genes, the DNA sequence of catalase genes CAT (rs1001179), glutathione-S-transferase GSTP1 (rs1695), glutathione peroxidase-4 GPX4 (rs713041) was used as the primers. Results. Correlative relationships between separate polymorphisms of genes and indicators of biochemical analysis of blood were found. For example, a polymorphic type of the gene GPX4 is associated with the lowest level of activity of glutathione reductase in heterozygotes, the activity of alkaline phosphatase is linked with the GSTP1 gene polymorphism, the level of catalase activity in blood serum correlates with the presence of a polymorphic type of the gene catalase CAT. Conclusions. Identification of candidate genes in patients with HCV is predictive for changes in the activity of several enzymes of the antioxidant system (GR, catalase) and enzymes that characterize toxic liver damage (AST, gamma-GTP), and therefore allows to predict the course of HCV in these patients.