Morphological Evaluation Of Effectiveness Of Electochemical Method With Nanotechnological Biosensors Using For Alkaline Phosphotase Detection In Colorectal Carcinoma Biopsies

Objective: To evaluate (morphologically) the effectiveness of the electrochemical method with biosensors applying to study the content of ALP in biopsies of tumors and intact colon mucosa. Materials and methods: Using the electrochemical method with nanotechnological biosensors, the concentration of alkaline phosphatase in biopsy specimens of colorectal carcinomas and intact colon mucosa, obtained from endoscopy from 24 patients were examined. Based on the results of a subsequent histological and immunohistochemical study of biopsy specimens, the effectiveness of the electrochemical method was evaluated. Results: The average value of the current, obtained in the study of fresh tumor biopsies was 0.0492 ± 0. 0007 μA, in case of biopsy specimens of the intact colon mucosa was 0.1197 ± 0.019 μA. Similar results were obtained in the study of biopsies, fixed in formalin: tumor tissue - 0.033 ± 0.0005 μA, intact mucosa - 0.0596 ± 0.0008 μA. The histological structure of tumors corresponded to adenocarcinoma of various degrees of differentiation. A positive correlation of the results of electrochemical and histological / immunohistochemical studies was observed in all patients. Immunohistochemical study of biopsy specimens with antibodies to non-specific alkaline phosphatase revealed high expression of enzyme in the cell membranes of the glandular crypt’s colon epithelium and a weak “background” staining of the cytoplasm and cell membranes of tumor cells. Conclusion: An electrochemical study with biosensors can be used to assess the alkaline phosphatase content in tumor biopsies and intact colon mucosa. Differences in the content of alkaline phosphatase in the tissue of colon adenocarcinoma and the unmodified mucosa are statistically significant. The existing differences in the study of fresh and fixed in formalin biopsy samples indicate the advisability of non-fixed material using.

колоректальный рак, щелочная фосфатаза, электрохимический метод, биочипы, colorectal cancer, alkaline phosphatase, electrochemical method, biochips