MICROBIOCENOSIS OF UPPER GASTROINTESTINAL TRACT IN PATIENTS WITH GASTROESOPHAGEAL REFL UX DISEASE, ASSOCIATED WITH BILIARY PATHOLOGY

Abstract

Aim. The study of the qualitative and quantitative characteristics of microbiocenosis of the oral cavity and esophagogastroduodenal zone in patients with gastroesophageal reflux disease (GERD), associated with duodenogastroesophageal reflux (DGER) as a manifestation of biliary pathology. Materials and methods. In 83 GERD patients with symptoms of reflux esophagitis and DGER we studied the microbial landscape of the oral fluid and biopsy samples from visually unaltered sections of the mucosa of the lower third of the esophagus, antrum and duodenum by the classical bacteriological methods. Results. Biopsy specimens from the esophagogastroduodenal zone mucosa in patients with GERD indicated high content of staphylococci (92 %) and streptococci (85 %) in amount of from 4,8 to 6,1 lg CFU/g. Bacteria of the Enterobacteriaceae family were defined in 61 % of cases in an amount of 3,1-5,4 lg CFU/g. Fungi of the genus Candida were detected in 59 % of the samples in the amount of 10⁴-10⁷ CFU. Bacteria characterized by cytotoxic, hemolytic and lecithinase activity were often detected in comparison with healthy people. Conclusion. The study revealed pathological microbiocenosis of upper gastrointestinal tract with opportunistic pathogens presence with factors of pathogen and cytotoxicity in patients with GERD associated with DGER, that define chronic inflammatory changes in the mucous membranes.