EFFECTS OF OZONE ON FREE RADICAL OXIDATION IN ACUTE OBSTRUCTIVE CHOLESTASIS

Abstract

Objective: The goals of this investigation is to study experimentally the effectiveness of intravenous infusions of ozonated 0.9 % sodium chloride solution for the correction of free-radical oxidation processes in acute obstructive cholestasis. Materials and methods: In 40 dogs under thiopental sodium (0.045-0.050 g / kg body weight) anesthesia was simulated a mechanical jaundice with the applying a loop on the common bile duct. Animals were divided into 4 series. In the first and second series after the simulation of cholestasis the common bile duct was decompressed on the third day. In the 3rd and 4th series after the decompression of the common bile duct for 4 days it were 0.9 % chloride and ozonized isotonic sodium chloride solution (OIS) infused. The pro- and antioxidant status were measured with the help of the luminescence induced plasma chemiluminescence intensity (Imax, S) in homogenates of the pancreas, the determination of the level of malondialdehyde (MDA) and catalase. Results. When ligation common bile duct the total bilirubin level was 40.3 times higher on the 3rd day of the experiment than in baseline. Imax index was increased by 2.7 times, chemiluminescence light sum - by 2.6 times of the original value. When ozone intravenous infusion for 7 days the maximal chemiluminescence intensity was decreased by 1.7 times and S parameter - by 68 %. Conclusion. Progressive obstructive jaundice in animals was accompanied by an increase in the intensity of free radical processes. But the infusions OIS followed by a significant decrease in the intensity of free radical processes that expressed by the decreasing in the amount of lipid peroxidation and was accompanied by increasing of antioxidant enzymes activity.