COMPOSITION OF FATTY ACIDS IN HUMAN SUBCUTANEOUS ADIPOSE TISSUE AS AN INDICATOR OF GASTROINTESTINAL DISEASES
Abstract
The Purpose. To analyze the composition of human subcutaneous adipose tissue in healthy individuals for the amount and composition of fatty acids. Material and methods. 10 samples of subcutaneous adipose tissue of different individuals from different anatomical regions have been studied. Adipose tissue was collected during liposuction from the abdomen, lumbar region and hips as the different samples, then it was centrifuged. The subject of the analysis was the upper part of lipoaspirate - pure fat that was released from the destroyed adipocytes. Pure fat samples were centrifuged and methylated by NaOH in methanol solution. Methyl esters were extracted by hexane and MTBE then analyzed on GC-MS Thermo Scientific Trace GC Ultra DSQ. The material was examined for 27 fatty acids. Results. In samples dominated fat oleic acid methyl ester C18:1n9c (42.3±2.2%) and palmitic acid C16:0 (22.5±3.4%). In less percent linoleic C18:2 (17.5±6.5%), stearic C18:0 (4.8±1.0%), palmitoleic C16:1t (3.0±0.6%), myristic C14:0 (2.2±0.5%) and eicosenoic C20:1n9 (0.8±0.2%) acids observed. It was found that the ratio of fatty acids in each sample was the same, they were different only in total amount. Conclusions. In human subcutaneous fatty tissue, regardless of the anatomic zones are more common oleic, methyl ester, palmitic, stearic and linoleic acids.
About the Authors
N. I. Khramtsova
Perm State University of Medicine named after academician E. A. Wagner of the Ministry of Healthcare of Russia
Russian Federation
Russian Federation
S. A. Plaksin
Perm State University of Medicine named after academician E. A. Wagner of the Ministry of Healthcare of Russia
Russian Federation
Russian Federation
References
1. Di Caro S., Cheung W. H., Fini L. Et al. Role Of Body Composition And Metabolic Dysfunction In Barrett’s Oesophagus And Progression To Cancer // Eur J Gastroenterol Hepatol. - 2015 Dec 14 [Epub ahead of print].
2. Wang C., Li J. Pathogenic Microorganisms and Pancreatic Cancer // Gastrointest Tumors. 2015 May;2(1):41-7.
3. Daniel C. R., Shu X., Ye Y., Gu J. et al. Severe obesity prior to diagnosis limits survival in colorectal cancer patients evaluated at a largecancer centre // Br J Cancer. - 2015 Dec 17. [Epub ahead of print].
4. Carnielli V. P., Sulkers E. J., Moretti C. et al. Conversion of octanoic acid into long-chain saturated fatty acids in premature infants fed a formula containing medium-chain triglycerides // Metabolism. - 1994. - Oct;43(10):1287-92.
5. McKimmie R.L., Easter L., Weinberg R. B. Acyl chain length, saturation, and hydrophobicity modulate the efficiency of dietary fatty acid absorption in adult humans // Am J Physiol Gastrointest Liver Physiol. - 2013. - Nov;305(9).
6. Kim J. M., Lee K. P., Park S. J. et al. Omega-3 fatty acids induce Ca2+ mobilization responses in human colon epithelial cell lines endogenously expressing FFA4 // Lipids Health Dis. 2013 Jul 13;12:101.
7. Hlavaty P., Tvrzicka E., Stankova B. et al. Association of plasma lipids fatty acid composition with metabolic profile of Czech adolescents // Physiol Res. 2015;64 Suppl 2: S167-75.
8. Giuliani A. Adipose tissue fatty acid composition and colon cancer: a case-control study // Eur J Nutr. - 2014 Jun;53(4):1029-37.
9. Cottet V, Vaysse C, Scherrer ML et al. Fatty acid composition of adipose tissue and colorectal cancer: a case-control study // Am J Clin Nutr. - 2015 Jan;101(1):192-201.
10. Zhang C., Yu H., Shen Y. et al. Polyunsaturated fatty acids trigger apoptosis of colon cancer cells through a mitochondrial pathway // Arch Med Sci. 2015 Oct 12;11(5):1081-94.
11. Fisk H. L., West A. L., Childs C. E. et al. The use of gas chromatography to analyze compositional changes of fatty acids in rat liver tissue during pregnancy // J Vis Exp. 2014 Mar 13;(85).
12. Carrillo C., Cavia Mdel M., Alonso-Torre S. R. Antitumor effect of oleic acid; mechanisms of action: a review // Nutr Hosp. - 2012 - Nov-Dec;27(6):1860-5.
13. Mancini A., Imperlini E., Nigro E. et al. Biological and Nutritional Properties of Palm Oil and Palmitic Acid: Effects on Health // Molecules. 2015 Sep 18;20(9):17339-61.
14. Sampath H., Ntambi J. M. The fate and intermediary metabolism of stearic acid // Lipids. 2005 Dec;40(12):1187-91.
15. Santaren I. D., Watkins S. M., Liese A. D. et al. Serum pentadecanoic acid (15:0), a short-term marker of dairy food intake, is inversely associated with incident type 2 diabetes and its underlying disorders // Am J Clin Nutr. 2014 Dec;100(6):1532-40.
Review
For citations:
Khramtsova N.I., Plaksin S.A. COMPOSITION OF FATTY ACIDS IN HUMAN SUBCUTANEOUS ADIPOSE TISSUE AS AN INDICATOR OF GASTROINTESTINAL DISEASES. Experimental and Clinical Gastroenterology. 2016;(12):106-109. (In Russ.)
Views:
420
Email this article 