INTESTINAL MICROBIOTA IN CROHN’S DISEASE: THE COMPOSITION AND CONNECTION WITH THE CLINICAL COURSE OF THE DISEASE
Abstract
The aim of our study was to estimate the intestinal microbiota composition and its impact on the clinical course of Crohn’s disease (CD). Materials and methods. The study involved 34 patients with CD: women - 28 (79.1%), man - 6 (21.0%), aged 37,07 ± 13,58 years (18-68). Terminal ileitis detected - in 18 (52.94%), Ileocolitis - 13 (38.24%), colitis - in 3 (8.82%) patients. Mild CD was observed in 2 (5.88%) patients, moderate - in 12 (35.3%) and severe CD - in 20 (58.82%) patients. CD was diagnosed by using traditional clinical, laboratory, instrumental, histological data. Microbiota composition was evaluated by gas chromatography and mass-spectrometry of whole blood. Results. Deficiency of Bifidobacterium, Lactobacillus, excessive content of Firmicutes phylum (Eubacterium, Cl. Perfringes, Cl. Difficile, Staphilococcus, Bacillus cereus), bacteria of Proteobacterium (H. pylori), Bacterouides (B. fragilis), Actinobacteria (Nocardia) were observed in CD patients. There was significant correlation between the CD severity and the level of following bacteria: Cl. difficile (r = 0.53, p = 0.02), Lactobacillus (r = -0.91; p = 0.01); between the length of the intestinal lesions and the level of Bacillus cereus (r = 0.87; p = 0.03), Eubacterium (r = 0.49; p = 0.04), Lactobacillus (r = -0.52; p =.02). Conclusion. A dysbiosis was revealed in Crohn’s disease in the composition of Firmicutes bacteria, Actinobacterium and Proteobacterium: increasing the number of Eubacterium, Cl. Perfringes, Cl. Dificile, H.pylori with a deficit of Bifidobacterium and Lactobacillus. Indicators of CD severity were more dependent on the level of Firmicutes bacteria - the degree of CD activity directly correlated with the number of Eubacterium, Cl. difficile, and inversely correlated with the number of obligate flora; and the extent of intestinal lesions directly correlated with the level of Bacilllus cereus and Eubacterium. The CD clinical course was depended not only on the presence of conditionally pathogenic flora, but also on a lack of commensal bacteria.
About the Authors
A. A. Shipovskaya
Federal state budgetary educational institution of higher professional education “Petrozavodsk state University”
Russian Federation
Russian Federation
O. P. Dudanova
Federal state budgetary educational institution of higher professional education “Petrozavodsk state University”
Russian Federation
Russian Federation
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Review
For citations:
Shipovskaya A.A., Dudanova O.P. INTESTINAL MICROBIOTA IN CROHN’S DISEASE: THE COMPOSITION AND CONNECTION WITH THE CLINICAL COURSE OF THE DISEASE. Experimental and Clinical Gastroenterology. 2016;(12):49-54. (In Russ.)
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