Effect of high-fat diet in female rats on physiological, biochemical and hematological parameters in first generation offspring

Objective of the study. To evaluate the effect of a high-fat diet in female rats on body weight, visceral fat deposits, biochemical and hematologic parameters in first generation offspring in an experiment. Materials and Methods. A control group of females receiving a normal fat diet (NFD) and a group receiving a high-fat diet (HFD) participated in the experiment. The diet was implemented before pregnancy, during pregnancy and during lactation. Male offspring within the group were divided into subgroups receiving the NFD and HFD. Feeding of males was continued for 2 months. Results. Mean weight (g) of HFD females 280.3±14.9, NFD 302±16.7; (p<0.05). Mean weight (g) of offspring of male offspring of NFD/HFD 201,9±28,7; NFD/NFD 198,2±28; HFD/HFD 207,8±29,1; HFD/NFD 185,9±27,3; (p>0,05). No relationship was found between male body weight dynamics and maternal diet [F (1, 79) = 0.002554, P=0.9598]. The increase in body weight of males was due to their diet [F (39, 79) = 2.282, P=0.0010]. The visceral adipose tissue mass ratio was predominantly HFD/HFD (p<0.05). The hemoglobin level of HFD/NFD was 6% higher than that of HFD/HFD (p<0.05). Statistically significant differences in cholesterol levels were determined between NFD/HFD and HFD/NFD (p<0.05). LDH levels were 47% lower in NFD/NFD than HFD/HFD (p<0.05). TNF-α level in NFD/HFD was 6 times higher than HFD/NFD (p<0.05). Statistically significant differences in glycemia levels at 120 min were determined between HFD/NFD and NFD/HFD (p<0.05). The glycemia level at 120 min averaged 5.9±0.6 mmol/L in the HFD/NFD group and 7.1±1.24 mmol/L in the NFD/HFD group. Conclusion. No changes in body weight, dynamics of feed and water consumption, and intergroup differences in glycemia levels were observed in females and offspring of first-generation males derived from females on HFD. No inflammatory or metabolic changes were detected. It is reasonable to consider a number of other alternative models of obesity

fetal programming, obesity, high-fat diet, offspring, maternal nutrition

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