Crystalloscopic examination of saliva in children with reduced nutritional status
https://doi.org/10.31146/1682-8658-ecg-233-1-71-76
Abstract
Objective. Evaluation of crystalloscopy and tesigraphy results in children with reduced nutritional status and long-chain 3-hydroxyacyl-CoA fatty acid dehydrogenase deficiency in a 10-month-old patient. Materials and methods. The saliva crystallography technique is fast and cost-effective. In addition, it is non-invasive, which is of particular importance in pediatric practice. In our work, the following indicators of saliva crystalloscopy were evaluated: crystallinity, structural index, degree of facies destruction and clarity of the marginal protein zone in eight children with reduced nutritional status without confirmed acute and chronic diseases. Nutritional status was determined on the basis of anthropometric data on the Z-Score scale. The crystalloscopic method was based on the study of the shape, size, color and other characteristics of saliva facies crystals. The crystals themselves were obtained by dehydrating a drop of saliva on a plate and conducting thesigraphy for faster crystallization - additional administration of chemicals to the biological fluid. Results. Such indicators of thesigraphy as: the main thesigraphic coefficient, crystallinity, the degree of destruction of facies and the clarity of the marginal protein zone were evaluated. Conclusion. Pronounced shifts in crystallogenic properties were revealed in the crystallograms of blood serum in the studied hereditary enzymopathy, and activation of dehydration structuring of blood serum was also recorded, which shows an increase in crystallizability and the structural index. Similar to urine samples, a sharp increase in the degree of facies destruction was revealed in blood serum in comparison with the samples of healthy children’s biological fluids, which indicates the diagnostic significance of the crystalloscopic method. The crystalloscopic “pattern” of biological fluid (saliva) in children with reduced nutritional status is characterized by an insignificant increase in crystallizability, a significant increase in the structural index and the degree of facies destruction. The tesigraphic “pattern” of biological fluid (saliva) in children with reduced nutritional status is characterized by a significant decrease in the main tesigraphic coefficient, a significant increase in crystallinity, and the degree of facies destruction. It is necessary to clarify the reasons for the change in these parameters.
About the Authors
I. V. Sadovnikova
Privolzhsky Research Medical University
Russian Federation
A. K. Martusevich
Lobachevsky State University of Nizhny Novgorod
Russian Federation
A. S. Kisurina
Privolzhsky Research Medical University
Russian Federation
A. O. Kukava
Privolzhsky Research Medical University
Russian Federation
A. I. Khavkin
Research Clinical Institute of Childhood, Ministry of Health of the Moscow Region; Belgorod State Research University. Ministry of Science and Higher Education of the Russian Federation
Russian Federation
G. A. Li
Research Clinical Institute of Childhood, Ministry of Health of the Moscow Region
Russian Federation
References
1. Program for optimization of feeding of children of the first year of life in the Russian Federation: methodological recommendations. Moscow. Federal State Autonomous Institution “National Medical Research Center of Children’s Health” of the Ministry of Health of the Russian Federation. b. i., 2019. 112 p. (in Russ.)@@ Программа оптимизации вскармливания детей первого года жизни в Российской П78 Федерации: методические рекомендации / ФГАУ «НМИЦ здоровья детей» Минздрава России. - М.: б. и., 2019. - 112 с.
2. Antropova I.P., Gabinsky Ya.L. Crystallization of biofluid in a closed cell using saliva as an example. Clinical laboratory diagnostics. 1997;(8):36-38. (in Russ.)@@ Антропова И.П., Габинский Я.Л. Кристаллизация биожидкости в закрытой ячейке на примере слюны. Клиническая лабораторная диагностика. 1997. № 8. С. 36-38.
3. Martusevich A.K., Grishina A.A. Biocrystallomics: general concepts, methodology and research methods. Kirov State Medical Academy. 2009, pp. 1-20. (in Russ.)@@ Мартусевич А.К., Гришина А.А. Биокристалломика: общие представления, методология и методы исследования. Кировская государственная медицинская академия. 2009. с. 1-20.
4. Gromova S.N., Smetanina O.A., Petrov S.B., Kuklina S.A., Kolevatykh E.P., Falaleeva E.A. The comparison of cleaning and remineralizing properties of toothpastes with and without surface-active substances. Pacific Medical Journal. 2020;(2):29-33. (In Russ.) doi: 10.34215/1609-1175-2020-2-29-33.@@ Громова С.Н., Сметанина О.А., Петров С.Б., Куклина С.А., Колеватых Е.П., Фалалеева Е.А. Сравнение очищающих и реминерализующих свойств зубных паст с поверхностно-активными веществами и без них. Тихоокеанский медицинский журнал. 2020;(2):29-33. doi: 10.34215/1609-1175-2020-2-29-33.
For citations:
Sadovnikova I.V.,
Martusevich A.K.,
Kisurina A.S.,
Kukava A.O.,
Khavkin A.I.,
Li G.A.
Crystalloscopic examination of saliva in children with reduced nutritional status. Experimental and Clinical Gastroenterology. 2025;(1):71-76.
(In Russ.)
https://doi.org/10.31146/1682-8658-ecg-233-1-71-76
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