Features of metabolic changes in the liver and kidneys of laboratory animals under the influence of aluminum hydroxide in a subacute experiment

Aluminum is a toxic metal for living systems. Aluminum accumulation is associated with damage to target organs: the liver and kidneys. The pollutant induces biochemical dysfunctions in the body, but the actual process is not fully understood. The purpose of the study was to evaluate metabolic changes in the liver and kidneys of laboratory animals after exposure to aluminum hydroxide in a subacute experiment. For 2 months, rats of three experimental groups received an aqueous solution of aluminum hydroxide daily orally in various doses. At the end of the experiment, the blood serum of experimental animals was used to conduct biochemical studies. The activity of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase was determined; content of total protein, albumin; levels of uric acid, urea and creatinine. It was found that subacute exposure to aluminum hydroxide in various doses significantly increased disturbances in the liver and kidneys of experimental animals, with concomitant changes in the level of indicator enzymes and metabolites. The toxic effects of aluminum are due to the formation of reactive oxygen species and the generation of free radicals. The accumulation of aluminum in the liver of experimental animals leads to damage to hepatocellular cells and the bile duct. Increased production of free radicals, along with decreased excretory capacity of the nephrons, contributes to functional changes in the kidneys.

aluminum, toxic metals, metabolic disorders, liver, kidneys, experimental animals, biochemical research

1. Sadauskiene I., Liekis A., Staneviciene I. et al. Effects of long-term supplementation with aluminum or selenium on the activities of antioxidant enzymes in mouse brain and liver. Catalysts. 2020;10(5): 585. doi: 10.3390/catal10050585.

2. Othman M.S., Fareid M.A., Abdel Hameed R.S., Abdel Moneim A.E. The protective effects of melatonin on aluminum-induced hepatotoxicity and nephrotoxicity in rats. Oxidative Medicine and Cellular Longevity. 2020;2020: 1-12. doi: 10.1155/2020/7375136.

3. Krewski D., Yokel R.A., Nieboer E. et al. Human health risk assessment for aluminium, aluminium oxide, and aluminium hydroxide. Journal of Toxicology and Environmental Health. 2007;10(1): 1-269. doi: 10.1080/10937400701597766.

4. Baranets A.A., Prigorelov G.A., Khamrayev Ye.T. Study of aluminum accumulation in the brain of laboratory animals. In: Topical issues of natural sciences. Materials of the IV All-Russian scientific-practical conference with international participation. Ivanovo; 2019: 188-192. (in Russ.)@@ Баранец А.А., Пригорелов Г.А., Хамраев Е.Т. Исследование кумуляции алюминия в головном мозге лабораторных животных. В кн.: Актуальные вопросы естествознания. Материалы IV Всероссийской научно-практической конференции с международным участием. Иваново; 2019: 188-192.

5. Usmanova E.N., Fazlyeva A.S., Daukaev R.A. et al. Assessment of aluminum content in food products sold in the Republic of Bashkortostan. Occupational medicine and human ecology. 2019;4: 68-72. doi: 10.24411/2411-3794-2019-10049.@@ Усманова Э.Н., Фазлыева А.С., Даукаев Р.А. и соавт. Оценка содержания алюминия в реализуемых на территории Республики Башкортостан пищевых продуктах. Медицина труда и экология человека. 2019;4: 68-72. doi: 10.24411/2411-3794-2019-10049.6. Al-Hazmi M.A., Rawi S.M., Hamza R.Z. Biochemical, histological, and neuro-physiological effects of long-term aluminum chloride exposure in rats. Metab Brain Dis. 2021;36(3): 429-436. doi: 10.1007/s11011-020-00664-6

6. Hosseini S.M., Hejazian L.B., Amani R., Badeli N.S. Geraniol attenuates oxidative stress, bioaccumulation, serological and histopathological changes during aluminum chloride-hepatopancreatic toxicity in male Wistar rats. Environ Sci Pollut Res. 2020;27(16): 20076-20089. doi: 10.1007/s11356-020-08128-1.

7. Hosseini S.M., Moshrefi A.H., Amani R. et al. Subchronic effects of different doses of zinc oxide nanoparticle on reproductive organs of female rats: an experimental study.Int J Reprod BioMed. 2019;17(2): 107-118. doi: 10.18502/ijrm.v17i2.3988.

8. Al-Hazmi M.A., Rawi S.M., Hamza R.Z. Biochemical, histological, and neuro-physiological effects of long-term aluminum chloride exposure in rats. Metab Brain Dis. 2021;36(3): 429-436. doi: 10.1007/s11011-020-00664-6.

9. Balgoon M.J. Assessment of the protective effect of Lepidium sativum against aluminum-induced liver and kidney effects in albino rat. BioMed Research International. 2019;2019: 1-9. doi: 10.1155/2019/4516730.

10. Salem F.E.H. Syzygium cumini (L) Extract ameliorates aluminium chloride-induced acute hepatic and renal toxicity in rats. Egyptian Academic Journal of Biological Sciences. 2021;13(2): 73-87. doi: 10.21608/eajbsc.2021.196250.

11. El-Demerdash F.M., Baghdadi H.H., Ghanem N.F., Al Mhanna A.B. Nephroprotective role of bromelain against oxidative injury induced by aluminium in rats. Environmental Toxicology and Pharmacology. 2020;80: 103509. doi: 10.1016/j.etap.2020.103509.

12. Belaïd-Nouira Y., Bakhta H., Haouas Z. et al. Fenugreek seeds reduce aluminum toxicity associated with renal failure in rats. Nutr Res Pract. 2013;7(6): 466-474. doi: 10.4162/nrp.2013.7.6.466.

13. Park S.H., Lim J.O., Kim W.I. et al. Subchronic toxicity evaluation of aluminum oxide nanoparticles in rats following 28-day repeated oral administration. Biological Trace Element Research. 2021;1: 1-12. doi: 10.1007/s12011-021-02926-5.

14. Marza Hamza N., Malik Yasir S., Hussain A.M. Biological effects of aqueous extract of Laurus noboilis L. Leaves on some histological and immunological parameters in male rat liver affected by aluminum chloride. Arch Razi Inst. 2021;76(6): 1745-1753. doi: 10.22092/ari.2021.356361.1827.

15. Cheraghi E., Roshanaei K. The protective effect of curcumin against aluminum chloride-induced oxidative stress and hepatotoxicity in rats. Pharm Biomed Res. 2019;5(1): 11-18. doi: 10.18502/pbr.v5i1.761.

16. El-Demerdash F.M. Antioxidant effect of vitamin E and selenium on lipid peroxidation, enzyme activities and biochemical parameters in rats exposed to aluminium. Journal of Trace Elements in Medicine and Biology. 2004;18(1): 113-121. doi: 10.1016/j.jtemb.2004.04.001.

17. Mokrane N., Kharoubi O., Tahari F.Z. et al. The effect of Thymus vulgaris L. on renal and liver toxicity in wistar rats exposed to aluminum. Journal of Medicinal Plants Research. 2020;14(1): 13-23. doi: 10.5897/jmpr2019.6819.

18. Yousef M.I. Aluminium-induced changes in hemato-biochemical parameters, lipid peroxidation and enzyme activities of male rabbits: protective role of ascorbic acid. Toxicology. 2004;199(1): 47-57. doi: 10.1016/j.tox.2004.02.014.

19. Benzaid C., Tichati L., Trea F. et al. Rhamnus alaternus aqueous extract enhances the capacity of system redox defence and protects hepatocytes against aluminum chloride toxicity in rats. Clin Phytoscience. 2021;7(1): 1-9. doi: 10.1186/s40816-021-00302-3.

20. Al-Kahtani M., Abdel-Daim M.M., Sayed A.A. et al. Curcumin phytosome modulates aluminum-induced hepatotoxicity via regulation of antioxidant, Bcl-2, and caspase-3 in rats. Environmental Science and Pollution Research. 2020;27(17): 21977-21985. doi: 10.1007/s11356-020-08636-0.

21. Kinawy A.A. Potential toxicity of aluminum and fluoride on some biochemical aspects of male rat’s offspring. The Journal of Basic and Applied Zoology. 2019;80(1): 1-7. doi: 10.1186/s41936-019-0087-1.