The role of SNP×SNP interactions of polymorphic loci of candidate genes in the pathogenesis of duodenal ulcer

Introduction: Duodenal ulcer is the most common disease of the gastrointestinal tract with a prevalence of 4-15%, occurs 4 times more often in prevalence with peptic ulcer and occurs more often from 30 to 55 years. The contribution of hereditary factors to the etiopathogenesis of diseases is 5.5-50%. Objective: to evaluate the role of SNP×SNP interactions of polymorphic loci of candidate genes (rs2294008, rs505922, rs6136, rs8176720, rs2519093, rs507666, rs651007, rs579459, rs649129) in the development of duodenal ulcer (DU). Materials and methods: The sample consisted of 217 patients with DU and 347 individuals of the control group, the regulatory potential of polymorphic loci was evaluated using the online databases, genotyping was performed by PCR. The study of SNP×SNP interactions of polymorphic variants of candidate genes associated with the development of GU was carried out using a modification of the MDR (Multifactor Dimensionality Reduction) - Model-Based-MDR (MB-MDR) method, data visualization was carried out in the form of a dendrogram and graph using MDR software (v. 3.0.2). Results: Six out of 9 studied SNPs as part of 5 significant models of interlocus interactions are involved in the formation of duodenal ulcer. The largest number of models includes rs2294008 of the PSCA gene, rs8176720 and rs579459 of the ABO gene. These polymorphic variants have a pronounced regulatory potential in many organs (tissues), incl. in the organs of the digestive system.

duodenal ulcer, SNP×SNP interactions, intergenic interactions, polymorphic variants

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