Microbiocenosis of colon and ability to biofi lm formation of strains Klebsiella spp. in children with functional gastrointestinal disorders

Objective of the study: to establish the features of large intestine microbiocenosis in children with functional gastrointestinal disorders (FGIDs) and to analyze the biofi lm ability of Klebsiella spp. strains isolated in the study.

Materials and methods: the coprological tests from 83 children, divided into two groups: The fi rst group (n=34) is children under one year, the second group (n=49) is children over one year. The ability of biofi lm formation has been studied in 53 strains of Klebsiella spp. identifi ed in this study.

Results: It has been identifi ed that signifi cant factors in pathogenesis of FGIR in children are defi ciency indices of bifi dobacterial content, appearance of modifi ed Escherichia coli strains and increase of microbial density of dominant species of opportunistic bacteria — Klebsiella spp. and Staphylococcus aureus. Klebsiella was shown to have the ability to form biofi lm communities (48.1% in children under one year old and 50.0% in children over one year old). It is revealed that the presence of Klebsiella in the intestine is a signifi cant risk factor for the development of dysbiosis 2–3 degrees, and in the absence of Klebsiella spp. as part of the intestinal microbiocenosis in children, regardless of age, dysbiotic disorders of 1 degree (p<0,05) were recorded.

Conclusion: Persistence Klebsiella spp. with biofi lm formation ability has implications for the conservation and continued existence of associative pathological microbiocenosis that exacerbate bowel dysfunction.

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