Immunohistochemical markers CDX2, CK20, CK7 in the evaluation of severity lesions of the gastric mucosa in schoolchildren with gastritis
https://doi.org/10.31146/1682-8658-ecg-175-3-54-59
Abstract
The aim is study the association of CDX2, CK20, CK7 with morphological changes in the gastric mucosa at children of aboriginal and newcomer population of the Republic of Tuva with gastritis.
Materials and methods. We examined of children with gastroenterological complaints by 2 ethnic groups: 69 aboriginal and 34 immigrants. All underwent esophagogastroduodenoscopy with sampling of biopsies from the antral region and the body of the stomach. Diagnosis of gastritis was carried out according to the modified Sydney classification. H. pylori was determined of coloring the biopsies by Gimza. Expression of biomarkers was recorded by immunohistochemical method (CDX2, CK20, CK7). The analysis of the statistical significance of differences in qualitative characteristics was carried out using the criterion χ2.
Results. We had determined the expression of CDX2 in 4 schoolchildren in the Republic of Tuva. This is can be examined as a marker for dynamic observation with a poor prognosis, because marker CDX2 tight connect with metaplasia and atrophy in gastric mucosa at adults. Association of the expression of CK20 and CK7 was established with the activity of gastritis, infection H. pylori, metaplasia of gastric mucosa, what more expressed by Tuva population. This has been testifying of the intensification proliferative processes in the gastric mucosa. This fact explaining the acceleration of progression the inflammatory process into the body of stomach. All it’s associated with an increased risk of dystrophy and atrophy at the gastric mucosa.
Conclusion. CDX2 CK20, CK7 can to act as biomarkers of development the precancerous damage of stomach in schoolchildren of Tuva, especially by Tuvans. The presence of atrophy, metaplasia or CDX2 protein is a prognostically unfavorable factor.
About the Authors
V. A. Vshivkov
Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch of the Russian Academy of Sciences — Scientific Research Institute for Medical Problems of the North
Russian Federation
Russian Federation
Vitaly A. Vshivkov, Clinical Division of Digestive System Pathology in Adults and Children, Senior scientist, MD
Krasnoyarsk, 660022
T. V. Polivanova
Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch of the Russian Academy of Sciences — Scientific Research Institute for Medical Problems of the North
Russian Federation
Russian Federation
Tamara V. Polivanova, Clinical Division of Digestive System Pathology in Adults and Children, Chief scientist, MD
Krasnoyarsk, 660022
E. V. Kasparov
Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch of the Russian Academy of Sciences — Scientific Research Institute for Medical Problems of the North
Russian Federation
Russian Federation
Eduard V. Kasparov, Director, MD, Professor
Krasnoyarsk, 660022
O. V. Peretyatko
Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch of the Russian Academy of Sciences — Scientific Research Institute for Medical Problems of the North
Russian Federation
Russian Federation
Olga V. Peretyatko, Clinical Division of Digestive System Pathology in Adults and Children, Scientist
Krasnoyarsk, 660022
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Review
For citations:
Vshivkov V.A., Polivanova T.V., Kasparov E.V., Peretyatko O.V. Immunohistochemical markers CDX2, CK20, CK7 in the evaluation of severity lesions of the gastric mucosa in schoolchildren with gastritis. Experimental and Clinical Gastroenterology. 2020;(3):54-59. (In Russ.) https://doi.org/10.31146/1682-8658-ecg-175-3-54-59
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