Levels of neoangiogenesis factors in pathological tissues of gastric cancer patients

Abstract

The aim was to study levels of VEGFA, VEGFC, sVEGFR1, sVEGFR3 and TGFβ1 in malignant gastric, peritoneal and omental tissues. Materials and methods. 62 patients were enrolled in the study: 21 (10, 11) -stomach cancer T_3-4аN_0-3M₁ and metastases to the peritoneum; 24 (15, 9) - stomach cancer T_3-4аN_0-3M₀without metastasis; 17 (6, 11) - non-cancer patients (controls). Levels of growth factors and their receptors were measured by ELISA in tissues of the peritoneum, greater omentum, gastric tumors (GT) and their perifocal tissues (PT). Results. All patients had elevated GT and PT levels of VEGFА and VEGFR1, but not VEGFСand VEGFR3; GT tissues contained more VEGFR1, and PT - more VEGFА. Only metastatic peritoneum and omentum showed increased levels of VEGFs: in the omentum -VEGFА (by 2.8 times), VEGFС (by 2.7 times), VEGFR1 (by 2.3 times), VEGFR3 (by 1.6 times (p<0.05)); in the peritoneum - VEGFА (by 4.2 times0, VEGFС (by 3.5 times), VEGFR1 (by 2.5 times), VEGFR3 (by 2.3 times). The TGF-β1 level increased in GT, PT and omentum tissues in all patients despite metastases, while peritoneal TGF-β1 levels sharply increased - by 3.1 times with metastases and decreased - by 1.8 times (p<0.05) without ones. Conclusion. The role of VEGFА and TGFβ1 in omental and peritoneal metastasis from gastric cancer was confirmed.