Abstract
Aims: to identify the “marker” changes in the composition of intestinal microbiota in patients with disorders of carbohydrate metabolism. Materials and methods: to investigate the gut microbiota composition in association with the glucose tolerance analyzed 92 patients (Me 52,8 years): with normal glucose tolerance (n=48), prediabetes (n=24) and T2D (n=20). Metagenomic analysis was performed using 16SrRNA sequencing. Results: Firmicutes (68,2±11,9%) predominantly represented microbiota, in a less degree by Bacteroidetes (15,1±11,1%). Shannon diversity index was higher (3.71±0.56) regardless of the presence or absence of impaired carbohydrate metabolism. The representation of Firmicutes was higher (p=0,007), Bacteroidetes (p=0,01) was lower in T2D. Blautia was a dominant genus in all samples. The representation of Blautia, Serratia was lower in prediabetes than in T2D, and even lower in normal glucose tolerance. C-reactive protein and Interleukin-6 were associated with higher representation of Serratia and Paraprevotella (p<0,007). Taxonomic analysis of the faecal metagenomes revealed two fecotypes with an average silhouette value of 0.22. Subjects in each fecotype did’t differ according to sex distribution and age but differ according to high incidence of T2D (p=0.016) in fecotype where there was low index of alpha diversity (p=8.089e-05) and the representation of the enzymes that converts butyryl-CoA to butyrate. In clusters found differences according to the percentage of vitamin (B9, B2, B6, K, B12) synthesis pathway. Fecotype 1st is dominated Prevotella, Oscillospira, Flavobacterium, Sphingobacterium, Parabacteroides, while fecotype 2 by Ruminococcus, Peptoniphilus, Thiothrix, Legionella (p<0.004). Conclusions: study provides the further evidence concerning the structural modulation of the microbiota in the T2D pathogenesis.