Subpopulation composition of T-lymphocytes in patients with irritatable intestinal syndrome

A comparative study of differentiation of CD3+CD4+ peripheral blood lymphocytes of healthy volunteers (n=30) and patients with irritable bowel syndrome (IBS, n=22) was conducted using multicolored flow cytometry. In patients with IBS, The level of T-helpers of Central memory (CM), CD45RA-CD62L+ in patients with IBS significantly exceeded the values of the control group. Among the General population of CCR6+ CM Th17 there were significant increases in the percentage of DN Th17 (from 2.58% (1.55; 3.15) to 3.14% (2.53; 4.09) at p=0.011) and Th17.1 (from 9.42% (7,594 10.52) to 10.08% (10.33; 14,48) at p=0,002) when compared with the control. Moreover, IBS showed a change in the subpopulation composition of follicular T-helper cells (Tfh). Thus, in patients with IBS the relative content of Tfh2 cells and not yet described in detail Tfh with phenotype CXCR3+CCR6-CCR4+ decreased, while the level of two populations of Tfh with properties of Th17 (DN Tfh17 and Tfh17.1) significantly increased. The detected changes in the composition of Tfh suggest the presence of changes in the functioning of a specific humoral immune response in this disease. Consideration of the participation of these cells in the pathogenesis of IBS stimulation and differentiation in lymphocytes - the subject of further research. The revealed features of the subpopulation composition of T - lymphocytes complement the existing understanding of the mechanisms of occurrence of this disease and should be taken into account in the diagnosis, therapy and prevention of complications of this disease.

проточная цитометрия, синдром раздраженного кишечника, CD4+ лимфоциты, Т-хелперы 17, фолликулярные Т-хелперы, flow cytometry, irritable bowel syndrome, CD4+ lymphocytes, Th17 cells, follicular Th cells