Gastroprotective effect of Alflutop on diclofenac-induced gastropathy in rats

The aim of the study was to evaluate the gastroprotective effect of Alflutop solution for injection (K. O. Biotechnos S. A., Romania) on gastropathy induced by diclofenac in rats. Materials and methods: rats were treated with Alflutop (intramuscularly) and diclofenac (intragastrically) for 20 consecutive days. The levels of prostaglandins (PG) were estimated in stomach tissue homogenate supernatant using 4 groups, for the pathological study - 2 groups. Results: diclofenac administration resulted in development of lesions of the gastric mucosa. The 30% of animals treated with diclofenac and Alflutop did not demonstrate any pathological abnormalities, whereas pathological changes were observed in 100% of animals treated with diclofenac only. The decrease in the frequency and severity of desquamation of the epithelium was detected in the animals treated with Alflutop and diclofenac compared with the diclofenac group. Measurement of PG in stomach tissue homogenates showed that diclofenac induced decrease of the PGE₂, PGF_2α and PGI metabolite - 6-keto-PGF_1α. After discontinuation of diclofenac, PG levels continued to decline. The decrease of PGF_2α and 6-keto-PGF_1α was most pronounced (up to 16 and 20 times). Alflutop treatment was found to normalize the gastric levels of PGE₂ and 6-keto-PGF_1α by the 41^th day of the experiment. Reduced by diclofenac PGF_2α level was reinstated in the Alflutop group on the 21^th day, on the 41^th day PGF_2α concentration exceeded the levels of the control group. Conclusion: thus, Alflutop therapy resulted in rapid recovery of PG synthesis which had been suppressed by diclofenac. The potential mechanisms of Alflutop gastroprotective effects are discussed.

Алфлутоп, язвенная болезнь, воспаление, крысы, Alflutop, ulcer, inflammation, rat