Parameters of the gelatinase B activity in the integument epithelium and the lamina propria of the gastric mucosa in experimental acetate ulcer in rats
L. N. Rogava,
N. V. Shesternina,
V. N. Povetkina,
V. A. Staravoytov,
I. A. Fastova,
T. V. Zameshnik,
E. I. Gubanova,
S. Yu. Dyachkova,
D. A. Popov https://doi.org/10.31146/1682-8658-ecg-192-8-150-153
Abstract
Objective: to determine the specifi c number and expression intensity of gelatinase B-positive cells in the gastric surface epithelium and lamina propria (cell component) in the area of the ulcerous defect in rats with experimental acetate ulcer of the stomach.
Materials and methods: 18 white Wistar rats were divided into three groups: initial (intact), experimental (modeling of an acetate ulcer) and control (falsely operated) in the experiment. Tissues were obtained from the ulceration zone or the presumed ulceration zone (in the initial and control groups) to asses morphological changes and also indicators, characterizing the activity of gelatinase B 7 days after the simulation of the experiment.
Results and Discussion. It was revealed some increase in the specifi c number of gelatinase B-positive cells against the background of moderate accumulation of lymphocytes in the integument epithelium of the gastric mucosa. A signifi cant increase in the specifi c number of gelatinase B-positive cells at the maximum intensity of its expression and a moderate accumulation of eff ector cells of tissue alterations was determined in the cellular component of the lamina propria of the gastric mucosa at the same time.
About the Authors
L. N. Rogava
Federal State Government- Financed Educational Institution of Higher Education “Volgograd State Medical University” of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
Lyudmila N. Rogova, MD, professor, Professor of the Department of pathophysiology, clinical pathophysiology
1, Pavshikh Bortsov Sq., Volgograd, 400131
N. V. Shesternina
Federal State Government- Financed Educational Institution of Higher Education “Volgograd State Medical University” of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
Nataliya V. Shesternina, Cand. of Med. Sci., Associate Professor of the Department of pathophysiology, clinical pathophysiology
1, Pavshikh Bortsov Sq., Volgograd, 400131
V. N. Povetkina
Federal State Government- Financed Educational Institution of Higher Education “Volgograd State Medical University” of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
Victoria N. Povetkina, Cand. of Med. Sci., Associate Professor of the Department of pathophysiology, clinical pathophysiology
1, Pavshikh Bortsov Sq., Volgograd, 400131
V. A. Staravoytov
Federal State Government- Financed Educational Institution of Higher Education “Volgograd State Medical University” of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
Vladislav A. Staravoitov, Cand. of Med. Sci., Associate Professor of the Department of pathophysiology, clinical pathophysiology
1, Pavshikh Bortsov Sq., Volgograd, 400131
I. A. Fastova
Federal State Government- Financed Educational Institution of Higher Education “Volgograd State Medical University” of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
Irina А. Fastova, Cand. of Med. Sci., Associate Professor of the Department of pathophysiology, clinical pathophysiology
1, Pavshikh Bortsov Sq., Volgograd, 400131
T. V. Zameshnik
Federal State Government- Financed Educational Institution of Higher Education “Volgograd State Medical University” of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
Tatyana V. Zameshnik, Cand. of Med. Sci., Associate Professor of the Department of pathophysiology, clinical pathophysiology
1, Pavshikh Bortsov Sq., Volgograd, 400131
E. I. Gubanova
Federal State Government- Financed Educational Institution of Higher Education “Volgograd State Medical University” of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
Ekaterina I. Gubanova, MD, professor, Professor of the Department of pathophysiology, clinical pathophysiology
1, Pavshikh Bortsov Sq., Volgograd, 400131
S. Yu. Dyachkova
Federal State Government- Financed Educational Institution of Higher Education “Volgograd State Medical University” of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
Svetlana Yu. Dyachkova, Cand. of Med. Sci., Associate Professor of the Department of pathophysiology, clinical pathophysiology
1, Pavshikh Bortsov Sq., Volgograd, 400131
D. A. Popov
Federal State Government- Financed Educational Institution of Higher Education “Volgograd State Medical University” of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
Dmitry A. Popov, Cand. of Med. Sci., Associate Professor of the Department of pathophysiology, clinical pathophysiology
1, Pavshikh Bortsov Sq., Volgograd, 400131
References
1. Khasigov P. Z., Podobed O. V., Ktzoeva S. A., et al. Matrix metalloproteinase of normal human tissues. Biochemistry (Moscow). 2001. Vol. 66, no.2, pp. 130–140. (in Russ.)
2. L. N. Rogova, V. N. Povetkina, N. V. Grigoryeva, et аl. Vliyanie stressa na formirovanie eksperimental’noj acetatnoj yazvy u stressustojchivyh i stressneustojchivyh krys [Th e eff ects of stress on experimental acetate ulser formation in stress tolerant ans stress non-resistant rats]. Journal of VolgSMU, 2014;4(52):112–115. (in Russ.)
3. Rogova L. N., Shesternina N. V., Zabolotneva K. O., Staravojtov V. A. Agressivnye faktory i mekhanizmy zashchity tkanej zheludochno- kishechnogo trakta, ih rol’ v patologii. [Aggressive factors and mechanisms of protection of gastrointestinal tract tissues, their role in pathology]. Volgograd, VolgGMU Publ., 2014. 136 p.
4. Turna A. A. Aktivnost’ matriksnyh metalloproteinaz pri razlichnyh patogeneticheskih variantah vospaleniya. Diss. dokt. med. nauk [Activity of matrix metalloproteinases in various pathogenetic variants of infl ammation Dr. med. sci. diss]. Moscow, 2010. 225 p.
5. Trubitsyna I. E., Stvolinsky S. L., Kulikova O. I., et al. Protective eff ect of the new compound – salicylcarnosine – on the formation of chronic acetate gastric ulcer in rats. Experimental and Clinical Gastroenterology. 2019;163(3): 58–64. (In Russ.) DOI: 10.31146/1682– 8658-ecg-163–3–58–64
6. Okabe S, Amagase K. An overview of acetic acid ulcer models – the history and state of the art of peptic ulcer research. Biol Pharm Bull. 2005 Aug;28(8):1321–41. doi: 10.1248/bpb.28.1321. PMID: 16079471
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For citations:
Rogava L.N., Shesternina N.V., Povetkina V.N., Staravoytov V.A., Fastova I.A., Zameshnik T.V., Gubanova E.I., Dyachkova S.Yu., Popov D.A. Parameters of the gelatinase B activity in the integument epithelium and the lamina propria of the gastric mucosa in experimental acetate ulcer in rats. Experimental and Clinical Gastroenterology. 2021;(8):150-153. (In Russ.) https://doi.org/10.31146/1682-8658-ecg-192-8-150-153
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