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Differences in fatty acid profi les of erythrocyte membranes related to tumor localization in colorectal cancer (pilot study)

https://doi.org/10.31146/1682-8658-ecg-182-10-56-64

Abstract

Aim of work is to study the compositional features of fatty acids (FA) of erythrocyte membranes in patients with diff erent localization of the tumor in colorectal cancer (CRC).

Materials and methods. Using a chromatography-mass spectrometric system (GC/MS) Agilent 7000B based on three quadrupoles (USA), the composition of erythrocyte (Er) membranes of 129 patients with CRC was studied: (average age 63.2±9.4 years, of which 68 men and 61 women; 25 with proximal, 98 with distal tumor localization) and 35 people in the comparison group.

Results. A greater degree of decrease in the levels of saturated, monounsaturated FAs (MUFAs), and, conversely, an increase in the levels of polyunsaturated FAs (PUFAs) in patients with distal tumor localization compared to healthy ones, was established than the same ratio in the pair “proximal localization of tumor RCC — healthy”. A greater degree of increase in the level of omega-3 PUFAs was noted than omega-6, which aff ected the ratio n-6 / n-3, which was signifi cantly reduced in cancer patients, to a greater extent with distal tumor localization.
The most signifi cant for distinguishing tumors located in diff erent parts of the intestine were: saturated fatty acids — myristic C14:0 (p<0,001) and pentadecanoic C15:0 (p=0,012), omega-3 a-linolenic (C18:3; n-3) (p=0,02), whose levels were signifi cantly higher and, conversely, most of the omega-6 PUFAs (C18:2 n-6, C20:3 n-6, C20:4 n-6) and one omega-3 PUFAs — C22:6 n-3 (p<0,05), whose levels were signifi cantly lower with proximal localization of the tumor than with distal.

Conclusion. The results obtained indicate the importance of taking into account the localization of the tumor in patients with CRC when conducti ng studies of metabolic profiles. 

About the Authors

M. V. Kruchinina
Research Institute of Internal and Preventive Medicine — Branch of the Institute of Cytology and Genetics, SB RAS; Federal State Budget Educational Institution of Higher Education “Novosibirsk State Medical University” Ministry of Health of the Russian Federation
Russian Federation
Laboratory of Gastroenterology, Leading researcher, MD; Department of Propaedeutics of Internal Medicine, Associate Professor

630089, Novosibirsk, st. Boris Bogatkov, 175/1

630091, Novosibirsk, Krasnyj prospect, 52



I. O. Svetlova
Federal State Budget Educational Institution of Higher Education “Novosibirsk State Medical University” Ministry of Health of the Russian Federation
Russian Federation

Department of Therapy, Hematology and Transfusiology (Faculty of Continuing Education and Retraining of Doctors), PhD

630091, Novosibirsk, Krasnyj prospect, 52



S. A. Kurilovich
Research Institute of Internal and Preventive Medicine — Branch of the Institute of Cytology and Genetics, SB RAS; Federal State Budget Educational Institution of Higher Education “Novosibirsk State Medical University” Ministry of Health of the Russian Federation
Russian Federation

Head of Laboratory of Gastroenterology, MD; Department of Therapy, Hematology and Transfusiology (Faculty of Continuing Education and Retraining of Doctors), Professor

630089, Novosibirsk, st. Boris Bogatkov, 175/1

630091, Novosibirsk, Krasnyj prospect, 52



M. V. Shashkov
Boreskov Institute of Catalysis SB RAS
Russian Federation

Researcher, PhD

630090, Novosibirsk, Lavrentiev Ave. 5



A. S. Sokolova
Novosibirsk Institute of Organic Chemistry SB RAS
Russian Federation

Researcher, PhD

630090, Novosibirsk, Lavrentiev Ave. 9



V. N. Kruchinin
Rzhanov Institute of Semiconductor Physics SB RAS
Russian Federation

Researcher, PhD

630090, Novosibirsk, Lavrentiev Ave. 13



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Review

For citations:


Kruchinina M.V., Svetlova I.O., Kurilovich S.A., Shashkov M.V., Sokolova A.S., Kruchinin V.N. Differences in fatty acid profi les of erythrocyte membranes related to tumor localization in colorectal cancer (pilot study). Experimental and Clinical Gastroenterology. 2020;(10):56-64. (In Russ.) https://doi.org/10.31146/1682-8658-ecg-182-10-56-64

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