nogrЭкспериментальная и клиническая гастроэнтерологияExperimental and Clinical Gastroenterology1682-8658«Global Media Technologies»nogr-633Research ArticleКЛИНИЧЕСКАЯ ГАСТРОЭНТЕРОЛОГИЯCLINICAL GASTROENTEROLOGYСОСТАВ МИКРОБИОТЫ КИШЕЧНИКА У ОТНОСИТЕЛЬНО ЗДОРОВЫХ ЖИТЕЛЕЙ МОСКВЫ И МОСКОВСКОЙ ОБЛАСТИ С ОЖИРЕНИЕМTHE COMPOSITION OF THE INTESTINAL MICROBIOTA FROM HEALTHY RESIDENTS OF MOSCOW AND MOSCOW REGION WITH OBESITYКаштановаД. А.KashtanovaD. A.dr.kashtanova@gmail.comТкачеваО. Н.TkachevaO. N.noemail@neicon.ruКотовскаяЮ. В.KotowskaYu. V.noemail@neicon.ruПопенкоА. С.PopenkoA. C.noemail@neicon.ruТяхтА. В.TyakhtA. V.noemail@neicon.ruАлексеевД. Г.AlekseevD. G.noemail@neicon.ruБойцовС. А.BoytsovS. A.noemail@neicon.ruФГБОУ ВО «Российский национальный исследовательский медицинский университет имени Н. И. Пирогова» Минздрава России ОСП Российский геронтологический научно-клинический центрРоссияPirogov Russian National Research Medical University. Russian Clinical Research Center for GerontologyRussian FederationФГАОУ ВО Московский физико-технический институтРоссияMoscow institute of physics and technologyRussian FederationФГБУ «Национальный медицинский исследовательский центр кардиологии» Министерства здравоохранения Российской ФедерацииРоссияNational Medical Research Center of Cardiology” of the Ministry of Healthcare of the Russian FederationRussian Federation201820062018062935Copyright © Каштанова Д.А., Ткачева О.Н., Котовская Ю.В., Попенко А.С., Тяхт А.В., Алексеев Д.Г., Бойцов С.А., 20182018Каштанова Д.А., Ткачева О.Н., Котовская Ю.В., Попенко А.С., Тяхт А.В., Алексеев Д.Г., Бойцов С.А.Kashtanova D.A., Tkacheva O.N., Kotowska Y.V., Popenko A.C., Tyakht A.V., Alekseev D.G., Boytsov S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.nogr.org/jour/article/view/633

Цель исследования - изучить взаимосвязь состава микробиоты кишечника с индексом массы тела и абдоминальным ожирением у лиц без клинических проявлений хронических заболеваний. Материалы и методы. В исследование были включены 92 жителя Москвы и Московской области, мужчин и женщин в возрасте от 25 до 76 лет без клинических проявлений хронических неинфекционных заболеваний, не получающие медикаментозной терапии, но с возможным наличием кардиоваскулярных факторов риска, в том числе ожирения. Все участники прошли тщательное предварительное обследование, включавшее физический осмотр, клинический и биохимический анализы крови, электрокардиографию, тредмил-тест, оценку наличия факторов сердечно-сосудистого риска, а также секвенирование вариабельных участков V3-V4 гена 16S рРНК микробиоты кишечника и анализ питания. Результаты. В ходе исследования было обнаружено, что в исследуемой когорте лиц условно-патогенные бактерии родов Serratia и Prevotella были в большей степени представлены в образцах доноров у пациентов с избыточной массой тела и/или абдоминальным ожирением. Амилолитические бактерии рода Oscillosipa в меньшей степени представлены у лиц с абдоминальным ожирением. Пробиотические бактерии рода Bifidobacterium, производящие КЦЖК были существенно в меньшей степени представлены у лиц, потребляющих много холестерина или этанола. Заключение. Дисбаланс микробиоты кишечника с повышением уровня условно-патогенных бактерий и снижением строго анаэробных амилолитических бактерий ассоциирован с наличием ожирения.

Purpose of the study. To study the association between the gut microbiota composition and body mass index and abdominal obesity in participants without clinical chronic diseases. Materials and methods. The study included 92 residents from Moscow and the Moscow region, men and women aged 25 to 76 years without clinical chronic non-communicable diseases are not receiving medication, but with the possible presence of cardiovascular risk factors, including obesity. All participants underwent a thorough preliminary examination, including physical examination, clinical and biochemical blood tests, electrocardiography, treadmill test, evaluation of the presence of the cardiovascular risk factors, as well as the sequencing of the variable regions of the V3-V4 16S rRNA gene of the gut microbiota and diet analysis. Results. The study revealed that opportunistic bacteria of the genera Serratia and Prevotella were largely represented in overweight patients and / or patients with abdominal obesity. Amylolytic bacteria of the genus Oscillosipa were less represented in individuals with abdominal obesity. Probiotic bacteria of the genus Bifidobacterium producing SCFA were significantly less represented in people who consumed a lot of cholesterol or ethanol. Conclusion. Gut microbiota imbalance with an increase in opportunistic bacteria level and a decrease in strictly anaerobic amylolytic bacteria is associated with obesity.

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The authors declare that there are no conflicts of interest present.