nogrЭкспериментальная и клиническая гастроэнтерологияExperimental and Clinical Gastroenterology1682-8658«Global Media Technologies»nogr-498Research ArticleКЛИНИЧЕСКАЯ ГАСТРОЭНТЕРОЛОГИЯCLINICAL GASTROENTEROLOGYИССЛЕДОВАНИЕ АССОЦИАЦИИ ПОЛИМОРФИЗМОВ ГЕНОВ ФЕРМЕНТОВ БИОТРАНСФОРМАЦИИ КСЕНОБИОТИКОВ EPHX1 И CYP1A1 С РАЗВИТИЕМ ОСТРОГО ПАНКРЕАТИТА У РУССКИХTHE INVESTIGATION OF ASSOCIATION POLYMORPHISMS OF XENOBIOTICS BIOTRANSFORMATION ENZYMES GENES EPHX1 AND CYP1A1 TO THE DEVELOPMENT OF ACUTE PANCREATITIS IN RUSSIANSСамгинаТ. А.SamginaT. A.tass@list.ruБушуеваО. Ю.BushuevaO. Y.noemail@neicon.ruНазаренкоП. М.NazarenkoP. M.noemail@neicon.ruПолониковА. В.PolonikovA. V.noemail@neicon.ruФГБОУ ВО «Курский государственный медицинский университет» Минздрава РоссииРоссияFederal public budgetary educational institution of higher education “Kursk State Medical University” of the Ministry of Healthcare of the RussiaRussian Federation2017201120170113437Copyright © Самгина Т.А., Бушуева О.Ю., Назаренко П.М., Полоников А.В., 20172017Самгина Т.А., Бушуева О.Ю., Назаренко П.М., Полоников А.В.Samgina T.A., Bushueva O.Y., Nazarenko P.M., Polonikov A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.nogr.org/jour/article/view/498

Цель - исследование влияния полиморфизмов H139R и I462V генов EPHX1 и CYP1A1 на развитие острого панкреатита (ОП) в русской популяции. Образцы цельной крови были собраны у 299 больных острым панкреатитом и 238 здоровых. Генотипирование полиморфизмов (rs2234922) гена EPHX1 и (rs1048943) гена CYP1A1 выполнено с помощью метода ПЦР с дискриминацией аллелей с помощью TaqMan-зондов. Было обнаружено, что аллель 139H (OR=1.34 95 % CI 1.03-1.75) ассоциирован с пониженным риском развития острого панкреатита, ассоциации полиморфизма I462V с развитием острого панкреатита не обнаружено, проведенный стратифицированный анализ влияния курения и генотипов полиморфизмов H139R и I462V генов EPHX1 и CYP1A1 на развитие острого панкреатита также не обнаружил различий в группах больных и здоровых.

The aim of this study was to investigate the relationship between polymorphisms H139R and I462V of genes EPHX1 and CYP1A1 in the development of acute pancreatitis (AP) in the Russian population. Whole blood samples were collected from 299 patients with acute pancreatitis and 238 healthy controls. Genotyping of the SNPs (rs2234922) EPHX1 gene and (rs1048943) CYP1A1 gene accomplished using PCR with allele discrimination using TaqMan-probes. It has been found that the allele 139H (OR = 1.34 95 % CI 1.03-1.75) associated with a reduced risk of acute pancreatitis. I462V polymorphism association with development of acute pancreatitis detected. Conducted stratified analysis of smoking and genotypes of polymorphisms H139R and I462V genes EPHX1 and CYP1A1 in the development of acute pancreatitis also found no differences in the groups of patients and healthy controls.

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The authors declare that there are no conflicts of interest present.