nogrЭкспериментальная и клиническая гастроэнтерологияExperimental and Clinical Gastroenterology1682-8658«Global Media Technologies»10.31146/1682-8658-ecg-224-4-54-63nogr-2759Research ArticleЭКСПЕРИМЕНТАЛЬНАЯ ГАСТРОЭНТЕРОЛОГИЯEXPERIMENTAL GASTROENTEROLOGYНейрокогнитивные и поведенческие эффекты экстракта ладанника шалфеелистного Cistus salviifolius L. при экспериментальном неалкогольном стеатогепатитеNeurocognitive and behavioural effects of sage-leaved rock-rose Cistus salviifolius L. extract in experimental non-alcoholic steatohepatitisПриходькоВ. А.PrikhodkoV. A.veronika.prihodko@pharminnotech.comОрляхинаД. А.OrlyakhinaD. A.noemail@neicon.ruПетроваВ. Д.PetrovaV. D.noemail@neicon.ruСемивеличенкоЕ. Д.SemivelichenkoE. D.noemail@neicon.ruПлискоГ. А.PliskoG. A.noemail@neicon.ruКаревВ. Е.KarevV. E.noemail@neicon.ruИвкинД. Ю.IvkinD. Yu.noemail@neicon.ruОковитыйС. В.OkovityiS. V.noemail@neicon.ruФедеральное государственное бюджетное образовательное учреждение высшего образования «Санкт-Петербургский государственный химико-фармацевтический университет» Министерства здравоохранения Российской ФедерацииРоссияSaint Petersburg State Chemical and Pharmaceutical UniversityRussian FederationООО «Полисан»РоссияPolysan LtdRussian FederationФГБУ Детский научно-клинический центр инфекционных болезней Федерального медико-биологического агентстваРоссияPediatric Research and Clinical Center for Infectious Diseases of the Federal Medical Biological AgencyRussian FederationФедеральное государственное бюджетное образовательное учреждение высшего образования «Санкт-Петербургский государственный химико-фармацевтический университет» Министерства здравоохранения Российской Федерации; Научно-клинический и образовательный центр гастроэнтерологии и гепатологии, федеральное государственное бюджетное образовательное учреждение высшего образования «Санкт-Петербургский государственный университет»РоссияSaint Petersburg State Chemical and Pharmaceutical University; Scientific Clinical and Educational Center of Gastroenterology and Hepatology, Saint Petersburg State UniversityRussian Federation202415092024045463Copyright © Приходько В.А., Орляхина Д.А., Петрова В.Д., Семивеличенко Е.Д., Плиско Г.А., Карев В.Е., Ивкин Д.Ю., Оковитый С.В., 20242024Приходько В.А., Орляхина Д.А., Петрова В.Д., Семивеличенко Е.Д., Плиско Г.А., Карев В.Е., Ивкин Д.Ю., Оковитый С.В.Prikhodko V.A., Orlyakhina D.A., Petrova V.D., Semivelichenko E.D., Plisko G.A., Karev V.E., Ivkin D.Y., Okovityi S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.nogr.org/jour/article/view/2759

Неалкогольный стеатогепатит (НАСГ) является ведущей причиной хронических заболеваний печени в современном мире. Помимо сопутствующих метаболических и сердечно-сосудистых заболеваний, НАСГ связан с увеличением распространенности нейрокогнитивных и психических расстройств. Ладанник шалфеелистный Cistus salviifolius L. (CS) - растение с широким спектром биологической активности, включая возможные психотропные и прокогнитивные эффекты. Учитывая вышеизложенное, настоящее исследование было направлено на изучение потенциальных терапевтических эффектов водного экстракта CS при поведенческих дисфункциях и нарушениях памяти, связанных с экспериментальным алиментарным/токсическим НАСГ у мышей. 90 мышей-самцов C57Bl/6 были рандомизированы на следующие группы: (1) Контроль: НАСГ + отсутствие лечения; (2) CS253: НАСГ + 253 мг·кг-1 м. т. экстракт CS; (3) CS506: НАСГ + 506 мг·кг-1 м. т. Экстракт КС. НАСГ вызывали в течение 3 месяцев, и препараты вводили перорально один раз в день в течение экспериментального периода. По данным тестов «Открытое поле», «Приподнятый крестообразный лабиринт» и «Черно-белая камера» обе дозы экстракта CS моделировали седативный эффект с возможным анксиогенным эффектом у мышей. Кроме того, экстракт CS облегчал дисфункцию пространственной памяти, но не влиял на память распознавания объектов. Возможные механизмы действия экстракта включают усиление кальций-зависимой нейрональной передачи сигналов, а также модуляцию центральной нейротрансмиссии γ-аминомасляной кислоты, ацетилхолина или моноаминов, что требует дальнейшего выяснения. Ключевые слова: Cistus salviifolius L., седативные средства, нарушения памяти, когнитивная дисфункция, тревога, неалкогольный стеатогепатит, поведенческое тестирование, мыши

Non-alcoholic steatohepatitis (NASH) is the leading cause of chronic liver disease in the modern world. Besides metabolic and cardiovascular comorbidities, NASH is associated with increased prevalence of neurocognitive and psychiatric disorders. Sage-leaved rock-rose Cistus salviifolius L. (CS) is a plant with a wide spectrum of biological activities including possible psychotropic and procognitive effects. In view of the above, the present study was aimed at exploring the potential therapeutic effects of a CS aqueous extract in behavioural and memory dysfunction associated with experimental murine alimentary/toxic NASH. 90 male C57Bl/6 mice were randomized into the following groups: (1) Control: NASH + no treatment; (2) CS253: NASH + 253 mg·kg-1 b. w. CS ex-tract; (3) CS506: NASH + 506 mg·kg-1 b. w. CS extract. NASH was induced over 3 months, and the drugs were administered orally q. d. during the experimental period. As assessed by the Open field, Elevated plus maze, and Light/dark box test, both doses of the CS extract induced sedation with possible anxiogenic effect in mice. In addition, the CS extract alleviated spatial memory dysfunction but had no effect on object recognition memory. Possible mechanisms behind the extract’s effects include the potentiation of calcium-dependent neuronal signaling as well as the modulation of central γ-aminobutyric acid, acetylcholine, or monoamine neurotransmission, which requires further elucidation.

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The authors declare that there are no conflicts of interest present.