nogrЭкспериментальная и клиническая гастроэнтерологияExperimental and Clinical Gastroenterology1682-8658«Global Media Technologies»nogr-193Research ArticleКЛИНИЧЕСКАЯ ГАСТРОЭНТЕРОЛОГИЯCLINICAL GASTROENTEROLOGYГЕНДЕРНЫЕ РАЗЛИЧИЯ В ТКАНЕВОМ КАСКАДЕ АКТИВАЦИИ ПЛАЗМИНОГЕНА И PAI-1 ПРИ ПЛОСКОКЛЕТОЧНОМ РАКЕ ПИЩЕВОДАGENDER DIFFERENCES IN TISSUE CASCADE OF ACTIVATION OF PLASMINOGEN AND PAI-1 IN ESOPHAGEAL SQUAMOUS CELL CARCINOMAКитО. И.KitO. I.noemail@neicon.ruФранциянцЕ. М.FrantsiyantsE. M.noemail@neicon.ruКозловаЛ. С.KozlovaL. S.super.gormon@yandex.ruКолесниковЕ. Н.KolesnikovE. N.noemail@neicon.ruПогореловаЮ. А.PogorelovaYu. A.noemail@neicon.ruЧеряринаН. Д.CheryarinaN. D.noemail@neicon.ruТаварянИ. С.TavaryanI. S.noemail@neicon.ruФГБУ «Ростовский научно-исследовательский онкологический институт» Минздрава РоссииРоссияRostov Research Institute of OncologyRussian Federation201620052016051621Copyright © Кит О.И., Франциянц Е.М., Козлова Л.С., Колесников Е.Н., Погорелова Ю.А., Черярина Н.Д., Таварян И.С., 20162016Кит О.И., Франциянц Е.М., Козлова Л.С., Колесников Е.Н., Погорелова Ю.А., Черярина Н.Д., Таварян И.С.Kit O.I., Frantsiyants E.M., Kozlova L.S., Kolesnikov E.N., Pogorelova Y.A., Cheryarina N.D., Tavaryan I.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.nogr.org/jour/article/view/193

Цель исследования: изучение uPA-АГ, uPA-акт, tPA-АГ, tPA-акт, PAI-1-АГ, PAI-1-акт в тканях плоскоклеточного рака пищевода у мужчин и женщин в сравнительном аспекте для уточнения вопросов патогенеза. Материалы и методы. Методом ИФА с использованием стандартных тест-наборов «ELISA» исследована опухолевая ткань плоскоклеточного рака пищевода, её перифокальная зона (19 мужчин, 8 женщин в менопаузе, 38 - 72 лет, st II, G2, T2-3N0-1M0-1). Результаты. В ткани плоскоклеточного рака пищевода у мужчин обе формы uPA увеличены от линии резекции, у женщин - только uPA-акт. В опухолях женщин снижен tPA-АГ, tPA-акт снижен в опухолях всех больных. В ткани плоскоклеточного рака мужчин, обе формы PAI-1 были выше, чем в аналогичной ткани у женщин. В перифокальной зоне мужчин tPA-АГ был ниже линии резекции, а у женщин - выше. tPA-акт повышен у всех, но у мужчин выше, чем у женщин. Соотношение tPA-АГ/tPA-акт в опухолях мужчин в 5,7 раз выше, чем у женщин, в перифокальной зоне снижено у всех, но у мужчин снижение более выражено. Обе формы PAI-1 повышены в злокачественных тканях всех больных, но в опухоли они преобладают у мужчин, в перитуморозной ткани PAI-1-АГ у мужчин выше, чем у женщин. Выводы. Установлены достоверные гендерные различия экспрессии uPA, tPA и PAI-1 в тканях плоскоклеточного рака пищевода. В ткани опухоли плоскоклеточного рака пищевода и её перифокальной зоне возможно наличие опухоль-ассоциированных uPA, PAI-1 и tPA у мужчин, uPA и PAI-1 - у женщин. Определение системы регуляции плазминогена в тканях плоскоклеточного рака пищевода можно использовать для выбора и индивидуализации методов послеоперационного лечения.

Aim: comparative analysis of uPA-Ag, uPA-act, tPA-Ag, tPA-act, PAI-1-Ag and PAI-1-act in tissues of esophageal squamous cell carcinoma (ESCC) in men (M) and women (W) to clarify some pathogenesis issues. Material and methods. Tumor tissue of ESCC and its perifocal area (19 M and 8 menopausal W aged 38 - 72 years, st II, G2, T2-3N0-1M0-1) were studied by ELISA using standard test kits. Results. ESCC tissue of M showed an increase in both uPA types from the resection line (RL), while in W only uPA-act was increased. tPA-Ag was decreased in tumors of W, tPA-act - in tumors of all patients. Levels of both PAI-1 types were higher in ESCC of M than in W. In M, tPA-Ag in perifocal area was lower than in RL, while in W, on the contrary, it was higher. tPA-Ag/tPA-act coefficient in M tumors was 5.7 times higher than in W; in perifocal area it was reduced in all patients, being at the same time in M lower than in W. Both PAI-1 types were increased in malignant tissues of all patients, prevailing in M tumors, and PAI-1-Ag in peritumoral tissue was higher in M than in W. Conclusions. Significant gender differences were found in expression of uPA, tPA and PAI-1 in tissues of esophageal squamous cell carcinoma. Presence of tumor-associated uPA, PAI-1 and tPA in men and uPA and PAI-1 in women is possible in tumor tissue of esophageal squamous cell carcinoma and its perifocal area. Determination of plasminogen regulation system in tissues of esophageal squamous cell carcinoma can be used for the selection and individualization of postoperative treatments.

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The authors declare that there are no conflicts of interest present.