nogrЭкспериментальная и клиническая гастроэнтерологияExperimental and Clinical Gastroenterology1682-8658«Global Media Technologies»10.31146/1682-8658-ecg-182-10-56-64nogr-1450Research ArticleКЛИНИЧЕСКАЯ ГАСТРОЭНТЕРОЛОГИЯCLINICAL GASTROENTEROLOGYРазличия в жирнокислотных профилях мембран эритроцитов, связанные с локализацией опухоли при колоректальном раке (пилотное исследование)Differences in fatty acid profi les of erythrocyte membranes related to tumor localization in colorectal cancer (pilot study)КручининаМ. В.KruchininaM. V.

630089, г. Новосибирск, ул. Бориса Богаткова, 175/1

Российская Федерация, 630091, г. Новосибирск, Красный проспект, 52

630089, Novosibirsk, st. Boris Bogatkov, 175/1

630091, Novosibirsk, Krasnyj prospect, 52

kruchmargo@yandex.ruСветловаИ. О.SvetlovaI. O.

Российская Федерация, 630091, г. Новосибирск, Красный проспект, 52

Department of Therapy, Hematology and Transfusiology (Faculty of Continuing Education and Retraining of Doctors), PhD

630091, Novosibirsk, Krasnyj prospect, 52

КуриловичС. А.KurilovichS. A.

Лаборатория гастроэнтерологии, заведующая лабораторией, д. м. н.; профессор кафедры терапии, гематологии и трансфузиологии (факультета повышения квалификации и  переподготовки врачей)

630089, г. Новосибирск, ул. Бориса Богаткова, 175/1

Российская Федерация, 630091, г. Новосибирск, Красный проспект, 52

Head of Laboratory of Gastroenterology, MD; Department of Therapy, Hematology and Transfusiology (Faculty of Continuing Education and Retraining of Doctors), Professor

630089, Novosibirsk, st. Boris Bogatkov, 175/1

630091, Novosibirsk, Krasnyj prospect, 52

ШашковМ. В.ShashkovM. V.

Аналитическая лаборатория, научный сотрудник, кандидат химических наук

630090, г. Новосибирск, пр. Академика Лаврентьева, 5

Researcher, PhD

630090, Novosibirsk, Lavrentiev Ave. 5

СоколоваА. С.SokolovaA. S.

Лаборатория физиологически активных веществ, научный сотрудник, кандидат химических наук

630090, г. Новосибирск, пр. Академика Лаврентьева, 9

Researcher, PhD

630090, Novosibirsk, Lavrentiev Ave. 9

КручининВ. Н.KruchininV. N.

научный сотрудник, кандидат химических наук

630090, г. Новосибирск, пр. Академика Лаврентьева, 13

Researcher, PhD

630090, Novosibirsk, Lavrentiev Ave. 13

Научно-исследовательский институт терапии и профилактической медицины —филиал ФГБНУ ФИЦ Институт цитологии и генетики СО РАН; ФГБОУ ВО Новосибирский государственный медицинский университет Минздрава РоссииРоссияResearch Institute of Internal and Preventive Medicine — Branch of the Institute of Cytology and Genetics, SB RAS; Federal State Budget Educational Institution of Higher Education “Novosibirsk State Medical University” Ministry of Health of the Russian FederationRussian FederationФГБОУ ВО Новосибирский государственный медицинский университет Минздрава РоссииРоссияFederal State Budget Educational Institution of Higher Education “Novosibirsk State Medical University” Ministry of Health of the Russian FederationRussian FederationФГБУН Институт катализа им. Г. К. Борескова СО РАНРоссияBoreskov Institute of Catalysis SB RASRussian FederationФГБУН Новосибирский институт органической химии им. Н. Н. Ворожцова СО РАНРоссияNovosibirsk Institute of Organic Chemistry SB RASRussian FederationФГБУН Институт физики полупроводников им. А. В. Ржанова СО РАНРоссияRzhanov Institute of Semiconductor Physics SB RASRussian Federation2020271020200105664Copyright © Кручинина М.В., Светлова И.О., Курилович С.А., Шашков М.В., Соколова А.С., Кручинин В.Н., 20202020Кручинина М.В., Светлова И.О., Курилович С.А., Шашков М.В., Соколова А.С., Кручинин В.Н.Kruchinina M.V., Svetlova I.O., Kurilovich S.A., Shashkov M.V., Sokolova A.S., Kruchinin V.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.nogr.org/jour/article/view/1450Цель работы

Цель работы. Изучить особенности состава жирных кислот (ЖК) мембран эритроцитов у пациентов с различной локализацией опухоли при колоректальном раке (КРР).

Материалы и методы

Материалы и методы. С помощью хромато-масс-спектрометрической системы (ГХ/МС)  на основе трех квадруполей Agilent 7000B (США) исследован состав ЖК мембран  эритроцитов (Эр) 129 пациентов с КРР: (средний возраст 63,2±9,4 лет, из них — 68 мужчин и 61 женщина; 25 — с проксимальной, 98 — с дистальной локализацией  опухоли) и 35 человек группы сравнения.

Результаты

Результаты. Установлена большая степень снижения уровней насыщенных, мононенасыщенных ЖК (НЖК, МНЖК), и, напротив, повышения уровня полиненасыщенных ЖК (ПНЖК) у пациентов с дистальной локализацией опухоли по сравнению со здоровыми, чем то же соотношение в паре «проксимальная локализация КРР опухоли — здоровые». Отмечена большая степень повышения уровня омега-3 ПНЖК, чем омега-6, что сказалось на соотношении n-6 / n-3, которое было значимо снижено у больных раком, в большей степени при дистальной локализации опухоли. Наиболее значимыми для различения опухолей, локализующихся в различных частях кишки,  оказались: насыщенные жирные кислоты — миристиновая С14:0 (р<0,001) и  пентадекановая С15:0 (р=0,012), омега-3 α-линоленовая (С18:3; n-3) (р=0,02), уровни которых были достоверно выше и, напротив, большая часть омега-6 ПНЖК (С18:2 n-6, С20:3 n-6, С20:4 n-6) и одна омега-3 ПНЖК — С22:6 n-3 (р<0,05), уровни которых оказались достоверно ниже при проксимальной локализации опухоли, чем при дистальной.

Заключение

Заключение. Полученные результаты свидетельствуют о важности учета локализации  опухоли у пациентов с КРР при проведении исследований метаболических профилей.

Aim of work is to study the compositional features of fatty acids (FA) of erythrocyte membranes in patients with diff erent localization of the tumor in colorectal cancer (CRC).

Materials and methods

Materials and methods. Using a chromatography-mass spectrometric system (GC/MS) Agilent 7000B based on three quadrupoles (USA), the composition of erythrocyte (Er) membranes of 129 patients with CRC was studied: (average age 63.2±9.4 years, of which 68 men and 61 women; 25 with proximal, 98 with distal tumor localization) and 35 people in the comparison group.

Results

Results. A greater degree of decrease in the levels of saturated, monounsaturated FAs (MUFAs), and, conversely, an increase in the levels of polyunsaturated FAs (PUFAs) in patients with distal tumor localization compared to healthy ones, was established than the same ratio in the pair “proximal localization of tumor RCC — healthy”. A greater degree of increase in the level of omega-3 PUFAs was noted than omega-6, which aff ected the ratio n-6 / n-3, which was signifi cantly reduced in cancer patients, to a greater extent with distal tumor localization.The most signifi cant for distinguishing tumors located in diff erent parts of the intestine were: saturated fatty acids — myristic C14:0 (p<0,001) and pentadecanoic C15:0 (p=0,012), omega-3 a-linolenic (C18:3; n-3) (p=0,02), whose levels were signifi cantly higher and, conversely, most of the omega-6 PUFAs (C18:2 n-6, C20:3 n-6, C20:4 n-6) and one omega-3 PUFAs — C22:6 n-3 (p<0,05), whose levels were signifi cantly lower with proximal localization of the tumor than with distal.

Conclusion

Conclusion. The results obtained indicate the importance of taking into account the localization of the tumor in patients with CRC when conducti ng studies of metabolic profiles. 

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The authors declare that there are no conflicts of interest present.