nogrЭкспериментальная и клиническая гастроэнтерологияExperimental and Clinical Gastroenterology1682-8658«Global Media Technologies»10.31146/1682-8658-ecg-175-3-80-86nogr-1290Research ArticleЭКСПЕРИМЕНТАЛЬНАЯ ГАСТРОЭНТЕРОЛОГИЯEXPERIMENTAL GASTROENTEROLOGYВлияние экстракта корневищ куркумы длинной в составе ректальных суппозиториев на показатели перекисного окисления липидов в толстом кишечнике при экспериментальной болезни КронаImpact of extracum of curcuma longa in a composition of rectal suppositoria on of lipid peroxidal oxidation indications in the thick intestiness in experimental Crohn’s diseaseОсиковМ. В.OsikovM. V.

Осиков Михаил Владимирович, заведующий кафедрой патологической физиологии, профессор, д. м. н.

ул. Воровского, 64, Челябинск, Челябинская область, 454092

Mikhail V. Osikov, Head of the Department of Pathophysiology, professor, Doctor of Medical Sciences

Vorovskogo St., 64, Chelyabinsk, Chelyabinsk area, 454092

СимонянЕ. В.SimonyanE. V.

Симонян Елена Владимировна, заведующий кафедрой фармации и химии фармацевтического факультета, доцент, кандидат фармацевтических наук

ул. Воровского, 64, Челябинск, Челябинская область, 454092

Elena V. Simonyan, Head of the Department of Pharmacy and Chemistry, Pharmaceutical Faculty, Ph.D.

Vorovskogo St., 64, Chelyabinsk, Chelyabinsk area, 454092

БакееваА. Е.BakeevaA. E.

Бакеева Алина Евгеньевна, лаборант кафедры химии и фармации фармацевтического факультета

ул. Воровского, 64, Челябинск, Челябинская область, 454092

Alina E. Bakeeva, Laboratory assisstant of the Department of Pharmacy and Chemistry, Pharmaceutical Faculty

Vorovskogo St., 64, Chelyabinsk, Chelyabinsk area, 454092

a.kurenkova01@yandex.ruФГБОУ ВО «Южно-Уральский Государственный Медицинский Университет» Минздрава РФРоссияSouth Ural State Medical University, Ministry of health of the Russian FederationRussian Federation202022062020038086Copyright © Осиков М.В., Симонян Е.В., Бакеева А.Е., 20202020Осиков М.В., Симонян Е.В., Бакеева А.Е.Osikov M.V., Simonyan E.V., Bakeeva A.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.nogr.org/jour/article/view/1290Цель исследования

Цель исследования. Провести сравнительный анализ влияния экстракта куркумы длинной и 5-аминосалициловой кислоты (5-АСК) в составе ректальных суппозиториев на клиническую картину и содержание продуктов перекисного содержания липидов (ПОЛ) в слизистой оболочке толстого кишечника при экспериментальной БК.

Материалы и методы

Материалы и методы: эксперимент выполнен на 70 белых нелинейных крысах линии Wistar. БК моделировали ректальным введением тринитробензосульфоновой кислоты, ректальные суппозитории применяли через 12 часов: с 5-АСК в дозе 50 мг, с 10% экстрактом куркумы длинной оригинального состава, массой 0,3 г каждая. На 3, 5 и 7 сутки БК определяли клинический статус по модифицированной шкале Disease activity index и содержание продуктов ПОЛ в слизистой оболочке толстого кишечника.

Результаты

Результаты. В динамике БК в толстом кишечнике увеличивается содержание первичных, вторичных и конечных продуктов ПОЛ в гептановой и изопропанольной фазах; тяжесть клинических признаков (снижение массы тела, диарея, кровь в каловых массах) нарастает по мере увеличения содержания продуктов ПОЛ. Применение при БК ректальных суппозиториев с экстрактом куркумы снижает выраженность клинических симптомов, уменьшает содержание продуктов ПОЛ преимущественно в изопропанольной фазе липидного экстракта толстого кишечника; установлена ассоциация между индексом клинической активности БК и содержанием продуктов ПОЛ в толстом кишечнике. Эффективность локального применения экстракта куркумы в составе ректальных суппозиториев сопоставима с эффективностью применения ректальных суппозиториев с 5-АСК по показателям индекса клинической активности и содержания продуктов ПОЛ в толстом кишечнике.

The aim

The aim. To conduct a comparative analysis of the effect of turmeric extract and 5-aminosalicylic acid (5-ASA) in the composition of rectal suppositories in clinical symptoms and the content of lipid peroxidation products in the mucous membrane of the large intestine in experimental Crohn’s disease (CD).

Materials and methods

Materials and methods. The experiment was performed on 70 non-linear white Wistar rats. CD was modeled by rectal administration of trinitrobenzenesulfonic acid, rectal suppositories were used each 12 hours: with 5-ASA in a dose of 50 mg, with 10% turmeric extract of original composition, weighing 0.3 g each. On the 3rd, 5th and 7th day of CD, the clinical status was determined by the modified Disease activity index scale and the content of lipid peroxidation products in the large intestine mucosa.

Results

Results. In the dynamics of CD in the colon, the content of primary, secondary and final lipid peroxidative products in the heptane and isopropanol phases increases; the severity of clinical signs (weight loss, diarrhea, blood in the fecal masses) increases with increasing content of POL. The use of rectal suppositories with turmeric extract in case of CD reduces the severity of clinical symptoms, reduces the content of lipid peroxidation products mainly in the isopropanol phase of the colon lipid extract; an association was established between the index of clinical activity of CD and the content of lipid peroxidation products in the large intestine. The effectiveness of local application of turmeric extract in the composition of rectal suppositories is comparison with the effectiveness of the use of rectal suppositories with 5-ASA in terms of the clinical activity index and the content of lipid peroxidation products in the large intestine.

болезнь Кронаоксидативный стресспродукты перекисного окисления липидовэкстракт куркумыCrohn’s diseaseoxidative stresslipid peroxidation productsturmeric extractReferencesSales-Campos H., Basso P. J., Alves V. B.F. et. al. Classical and recent advances in the treatment of inflammatory bowel diseases. Braz J Med and Biol Res. 2014, no. 48(2), pp. 96–107.Sales-Campos H., Basso P. J., Alves V. B.F. et. al. Classical and recent advances in the treatment of inflammatory bowel diseases. Braz J Med and Biol Res. 2014, no. 48(2), pp. 96–107.Curkovic I., Egbring M., Kullak-Ublick G. Risks of Inflammatory Bowel Disease Treatment with Glucocorticosteroids and Aminosalicylates. Dig Dis, 2013, no.3, pp. 368–373Curkovic I., Egbring M., Kullak-Ublick G. Risks of Inflammatory Bowel Disease Treatment with Glucocorticosteroids and Aminosalicylates. Dig Dis, 2013, no.3, pp. 368–373Коллектив авторов. Болезнь Крона у взрослых // Клинические рекомендации ассоциации колопроктологов России и Российской гастроэнтерологической ассоциации, 2016 – С. 11–22Klinicheskie rekomendatsii rossiskoi gastroenterologicheskoi assotsiatsii I assotsiatsii koloproktologov Rossii po diagno. [Clinical recommendations of Russian gastroenterological association and association of coloproctologists for diagnostics and treatment of Crohn’s disease]. Available at: http://www.gastro.ru/userfiles/R_Krona_2017.pdf (accessed 29 September 2019)Tian T., Wang Z., Zhang J. Pathomechanisms of Oxidative Stress in Inflammatory Bowel Disease and Potential Antioxidant Therapies. Oxid med cell longev. Vol. 2017, Article ID: 4535194Tian T., Wang Z., Zhang J. Pathomechanisms of Oxidative Stress in Inflammatory Bowel Disease and Potential Antioxidant Therapies. Oxid med cell longev. Vol. 2017, Article ID: 4535194Пайзуллаева З. К., Александров В. Б. Нейроэндокринные изменения при воспалительных заболеваниях и раке толстой кишки // Клин. мед, 2010, № 4, С. 56–60.Paizullaeva Z., Aleksandrov V. Neiroendokrinnye izmeneniya pri vospalitel’nyh zabolevaniyah i rake tolstoy kishki [Neuroendocrine changes in imflammatory disease and thick colon cancer]. Klinicheskaya meditsina – Clinical medicine, 2010, no. 4. pp. 56–60.Novak E. A., Mollen K. P. Mitochondrial dysfunction in inflammatory bowel disease. Front Cell Dev Biol, 2015. № 3. – P. 62.Novak E. A., Mollen K. P. Mitochondrial dysfunction in inflammatory bowel disease. Front Cell Dev Biol, 2015. № 3.   – P. 62.Te Velde A. A., Pronk I., de Kort F. et al. Glutathione peroxidase 2 and aquaporin 8 as new markers for colonic inflammation in experimental colitis and inflammatory bowel diseases: an important role for H2O2? Eur J Gastroent Hepat. 2008, no. 20(6), pp. 555–560.Te Velde A. A., Pronk I., de Kort F. et al. Glutathione peroxidase 2 and aquaporin 8 as new markers for colonic inflammation in experimental colitis and inflammatory bowel diseases: an important role for H2O2? Eur J Gastroent Hepat. 2008, no. 20(6), pp. 555–560.Schrader M., Fahimi H. D. Peroxisomes and oxidative stress. BBA. 2006, Vol. 1763(12), pp. 1755–1766.Schrader M., Fahimi H. D. Peroxisomes and oxidative stress. BBA. 2006, Vol. 1763(12), pp. 1755–1766.Beltran B., Nos P., Dasi F., et al. Mitochondrial dysfunction, persistent oxidative damage, and catalase inhibition in immune cells of naive and treated Crohn’s disease. Inflamm. Bowel Dis. 2010, no. 16(1), pp. 76–86.Beltran B., Nos P., Dasi F., et al. Mitochondrial dysfunction, persistent oxidative damage, and catalase inhibition in immune cells of naive and treated Crohn’s disease. Inflamm. Bowel Dis. 2010, no. 16(1), pp. 76–86.Hiller F., Besselt K., Deubel S. et. al. GPx2 induction is mediated through STAT transcription factors during acute colitis. Inflamm. Bowel Dis. 2015, no. 21(9), pp. 2078–2089.Hiller F., Besselt K., Deubel S. et. al. GPx2 induction is mediated through STAT transcription factors during acute colitis. Inflamm. Bowel Dis. 2015, no. 21(9), pp. 2078–2089.Iborra M., Moret I., Rausell F., et al. Role of oxidative stress and antioxidant enzymes in Crohn’s disease. Biochem. Soc. Trans. 2011, no. 39(4), pp. 1102–1106.Iborra M., Moret I., Rausell F., et al. Role of oxidative stress and antioxidant enzymes in Crohn’s disease. Biochem. Soc. Trans. 2011, no. 39(4), pp. 1102–1106.Prabhu V., Guruvayoorappan C. Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits. Int Immunopharmacol. 2014, no. 18(1), pp. 124–134.Prabhu V., Guruvayoorappan C. Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits. Int Immunopharmacol. 2014, no. 18(1), pp. 124–134.Socca E. A., Luiz-Ferreira A., de Faria F. M., et al. Inhibition of tumor necrosis factor-alpha and cyclooxigenase-2 by Isatin: a molecular mechanism of protection against TNBS-induced colitis in rats. Chem.-Biol. Interact. 2014, Vol. 209, pp. 48–55.Socca E. A., Luiz-Ferreira A., de Faria F. M., et al. Inhibition of tumor necrosis factor-alpha and cyclooxigenase-2 by Isatin: a molecular mechanism of protection against TNBS-induced colitis in rats. Chem.-Biol. Interact. 2014, Vol. 209, pp. 48–55.Ioannidis O., Varnalidis I., Paraskevas G. et. al. Nutritional modulation of the inflammatory bowel response. Digestion. 2011, Vol. 84(2), pp. 89–101.Ioannidis O., Varnalidis I., Paraskevas G. et. al. Nutritional modulation of the inflammatory bowel response. Digestion. 2011, Vol. 84(2), pp. 89–101.Senhaji N., Kojok K., Darif Y. et. al. The contribution of CD40/CD40L axis in inflammatory bowel disease: an update. Front Immunol, 2015. No. 6, pp. 529.Senhaji N., Kojok K., Darif Y. et. al. The contribution of CD40/CD40L axis in inflammatory bowel disease: an update. Front Immunol, 2015. No. 6, pp. 529.Wallace K., Zheng L., Kanazawa Y. et. al. Immunopathology of inflammatory bowel disease. World J Gastroenterol, 2014, no. 20(1), pp. 6–21.Wallace K., Zheng L., Kanazawa Y. et. al. Immunopathology of inflammatory bowel disease. World J Gastroenterol, 2014, no. 20(1), pp. 6–21.Muthupalani S., Ge Z., Feng Y., et al. Systemic macrophage depletion inhibits Helicobacter bilis-induced proinflammatory cytokine-mediated typhlocolitis and impairs bacterial colonization dynamics in a BALB/c Rag2–/– mouse model of inflammatory bowel disease. Infect Immun. 2012, Vol. 80(12), pp. 4388–4397.Muthupalani S., Ge Z., Feng Y., et al. Systemic macrophage depletion inhibits Helicobacter bilis-induced proinflammatory cytokine-mediated typhlocolitis and impairs bacterial colonization dynamics in a BALB/c Rag2–/– mouse model of inflammatory bowel disease. Infect Immun. 2012, Vol. 80(12), pp. 4388–4397.Гайдарова А. П., Корощенко Г. А., Айзман Р. И. Влияние куркумы и куркумина на углеводный обмен при аллоксан-индуцированном сахарном диабете у крыс // Современные проблемы науки и образования. – 2014. – № .5. – С. 597.Gajdarova A., Koroshchenko G., Aizman R. Influence of turmeric and crcumin on carbohydrate metabolism in alloxan-induced diabetic rats. Modern problems of science and education. 2014, no.5, pp. 597.Gupta S. C., Patchva S., Aggarwal B. B. Therapeutic roles of curcumin: lessons learned from clinical trials. AAPS J, 2013, no. 15(1), pp.195–218.Gupta S. C., Patchva S., Aggarwal B. B. Therapeutic roles of curcumin: lessons learned from clinical trials. AAPS J, 2013, no. 15(1), pp.195–218.Morris G. P., Beck P. L., Herridge M. S. et. al. Hapteninduced model of chronic inflammation and ulceration in the rat colon. Gastroenterology. 1989, Vol. 3, pp. 795–803.Morris G. P., Beck P. L., Herridge M. S. et. al. Hapteninduced model of chronic inflammation and ulceration in the rat colon. Gastroenterology. 1989, Vol. 3, pp. 795–803.Cooper, H.S., Murthy, S.N., Shah, R.S. et. al. Clinicopathologicstudy of dextran sulfate sodium experimental murine colitis. Lab. Invest, 1993, no. 69(2), pp. 238–249.Cooper, H.S., Murthy, S.N., Shah, R.S. et. al. Clinicopathologicstudy of dextran sulfate sodium experimental murine colitis. Lab. Invest, 1993, no. 69(2), pp. 238–249.Волчегорский И. А., Долгушин И. И. и др. Экспериментальное моделирование и лабораторная оценка адаптивных реакций организма. Челябинск, ЧелГПУ, 2000. 167 с.Volchegorskyi I. A., Dolgushin I. I. et al. Experimentalnoe modelirovanie i laboratornaya otsenka adaptivnyh reaktsyi organizma [Experimental modelling and laboratory estimating of adaptive reactions of organism]. Chelyabinsk, ChelGPU, 2000. 167 p.Guo, Y. L., Li, X. Z., & Kuang, C. T. Antioxidant Pathways and Chemical Mechanism of Curcumin. Adv Mat Res. 2011, Vol. 236–238, pp. 2311–2314.Guo, Y. L., Li, X. Z., & Kuang, C. T. Antioxidant Pathways and Chemical Mechanism of Curcumin. Adv Mat Res. 2011, Vol. 236–238, pp. 2311–2314.Anthwal A., Thakur B. K., Rawat M. S. et. al. Synthesis, characterization and in vitro anticancer activity of C-5 curcumin analogues with potential to inhibit TNF-alpha-induced NF-kappaB activation. BioMed Research International Volume 2014, Article ID524161, 10 pages URL: http://dx.doi.org/10.1155/2014/524161Anthwal A., Thakur B. K., Rawat M. S. et. al. Synthesis, characterization and in vitro anticancer activity of C-5 curcumin analogues with potential to inhibit TNF-alpha-induced NF-kappaB activation. BioMed Research International Volume 2014, Article ID524161, 10 pages URL: http://dx.doi.org/10.1155/2014/524161Gupta S. C., Tyagi A. K., Deshmukh-Taskar P. et. al. Downregulation of tumor necrosis factor and other proinflammatory biomarkers by polyphenols. Arch Biochem Biophys. 2014, Vol. 559, pp. 91–99.Gupta S. C., Tyagi A. K., Deshmukh-Taskar P. et. al. Downregulation of tumor necrosis factor and other proinflammatory biomarkers by polyphenols. Arch Biochem Biophys. 2014, Vol. 559, pp. 91–99.

The authors declare that there are no conflicts of interest present.